School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China.
Environ Sci Pollut Res Int. 2022 Oct;29(50):76298-76309. doi: 10.1007/s11356-022-21148-3. Epub 2022 Jun 6.
The application of ionic liquids with sawdust and fresh dairy manure was studied in composting. The degradation of organic matter (OM), dissolved organic matter (DOM), and lignocellulose was analyzed. The DOM decreased by 14.25 mg/g and 11.11 mg/g in experimental group (ILs) and control group (CK), respectively. OM decreased by 7.32% (CK) and 8.91% (ILs), respectively. The degradation rates of hemicellulose, lignin, and cellulose in ILs (56.62%, 42.01%, and 23.97%) were higher than in CK (38.39%, 39.82%, and 16.04%). Microbial community and carbohydrate-active enzymes (CAZymes) were analyzed based on metagenomics. Metagenomic analysis results showed that ionic liquids enriched Actinobacteria and Proteobacteria in composting. Compared with CK, the total abundance values of GH11, GH6, AA6, and AA3_2 in ILs increased by 13.98%, 10.12%, 11.21%, and 13.68%, respectively. Ionic liquids can improve the lignocellulosic degradation by regulating the environmental physicochemical parameters (temperature, pH, C/N) to promote the growth of Actinobacteria and Proteobacteria and carbohydrate-active enzymes (CAZymes) abundance. Therefore, ionic liquids are a promising additive in lignocellulosic waste composting.
研究了木屑和新鲜奶牛粪肥与离子液体在堆肥中的应用。分析了有机物（OM）、溶解有机物（DOM）和木质纤维素的降解。实验组（ILs）和对照组（CK）中 DOM 分别减少了 14.25mg/g 和 11.11mg/g。OM 分别减少了 7.32%（CK）和 8.91%（ILs）。ILs 中半纤维素、木质素和纤维素的降解率（56.62%、42.01%和 23.97%）高于 CK（38.39%、39.82%和 16.04%）。基于宏基因组学分析了微生物群落和碳水化合物活性酶（CAZymes）。宏基因组分析结果表明，离子液体在堆肥中富集了放线菌和变形菌。与 CK 相比，ILs 中 GH11、GH6、AA6 和 AA3_2 的总丰度值分别增加了 13.98%、10.12%、11.21%和 13.68%。离子液体可以通过调节环境理化参数（温度、pH、C/N）来提高木质纤维素的降解，促进放线菌和变形菌的生长和碳水化合物活性酶（CAZymes）的丰度。因此，离子液体是木质纤维素废物堆肥中很有前途的添加剂。
