Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece; Center for Bioanalysis & Omics (BIOMIC_AUTH), CIRI Center for Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, 57001 Thessaloniki, Greece.
Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Aug 1;1179:122852. doi: 10.1016/j.jchromb.2021.122852. Epub 2021 Jul 5.
A static headspace gas chromatography - mass spectrometry (HS-GC/MS) method was developed and optimized with the aim to be applied in the analysis of lavender essential oil. To obtain a comprehensive profile of the essential oil, the optimum HS-GC/MS method parameters were selected based on a Design of Experiments (DοE) process. Plackett-Burman experimental design was applied by utilizing seven parameters of the HS injection system. Incubation equilibration temperature and time, agitator's vortex speed, post injection dwell time, inlet temperature, split ratio and injection flow rate were screened to select the optimum conditions on the basis of the number and the intensity of the identified compounds. Other parameters, such as sample volume and dilution solvent ratio, were also examined to achieve a comprehensive profile in a chromatographic run of 55 min. With the obtained optimum method, more than 40 volatile compounds were identified in lavender's essential oils from different geographical regions in Greece. The method can be utilized for the quality assessment of lavender's essential oil and provide information on its characteristic aroma and discrimination among species based on the acquired GC-MS profiles.
建立并优化了静态顶空气相色谱-质谱法（HS-GC/MS），旨在应用于薰衣草精油的分析。为了获得精油的综合特征图谱，我们基于实验设计（DoE）过程选择了最佳的 HS-GC/MS 方法参数。利用顶空进样系统的七个参数，采用 Plackett-Burman 实验设计筛选了孵育平衡温度和时间、搅拌器涡旋速度、进样后驻留时间、进样口温度、分流比和进样流速，根据鉴定化合物的数量和强度选择最佳条件。还检查了其他参数，如样品量和稀释溶剂比，以在 55 分钟的色谱运行中实现全面的特征图谱。利用获得的最佳方法，从希腊不同地理区域的薰衣草精油中鉴定出 40 多种挥发性化合物。该方法可用于薰衣草精油的质量评估，并根据获得的 GC-MS 图谱提供有关其特征香气和物种区分的信息。
