Zhang Cuili, Chen Jinhui, Huang Weixia, Song Xiqiang, Niu Jun
Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, College of Forestry, Hainan University, Haikou, Hainan, China.
Engineering Research Center of Rare and Precious Tree Species in Hainan Province, College of Forestry, Hainan University, Haikou, Hainan, China.
Front Genet. 2021 Jul 2;12:692702. doi: 10.3389/fgene.2021.692702. eCollection 2021.
Drought stress is a bottleneck factor for plant growth and development, especially in epiphytic orchids that absorb moisture mainly from the air. Recent studies have suggested that there are complex transcriptional regulatory networks related to drought stress in In this study, the transcription and metabolite alterations involved in drought stress response in were investigated through RNA-seq and metabolomics. A total of 856 metabolites were identified from stressed and control samples, with 391 metabolites showing significant differences. With PacBio and Illumina RNA sequencing, 72,969 genes were obtained with a mean length of 2,486 bp, and 622 differentially expressed genes (DEGs) were identified. Correlation analysis showed 7 differential genes, and 39 differential metabolites were involved in interaction networks. The network analysis of differential genes and metabolites suggested that the pathways of purine metabolism and phenylpropanoid biosynthesis may play an important role in drought response in . These results provide new insights and reference data for culturally important medicinal plants and the protection of endangered orchids.
干旱胁迫是植物生长发育的瓶颈因素，对于主要从空气中吸收水分的附生兰花而言尤其如此。最近的研究表明，在[此处原文缺失相关植物名称]中存在与干旱胁迫相关的复杂转录调控网络。在本研究中，通过RNA测序和代谢组学研究了[此处原文缺失相关植物名称]干旱胁迫响应过程中的转录和代谢物变化。从胁迫和对照样本中总共鉴定出856种代谢物，其中391种代谢物表现出显著差异。通过PacBio和Illumina RNA测序，获得了72,969个基因，平均长度为2,486 bp，并鉴定出622个差异表达基因（DEG）。相关性分析显示7个差异基因，39个差异代谢物参与了相互作用网络。差异基因和代谢物的网络分析表明，嘌呤代谢和苯丙烷生物合成途径可能在[此处原文缺失相关植物名称]的干旱响应中起重要作用。这些结果为具有重要文化意义的药用植物和濒危兰花的保护提供了新的见解和参考数据。
