Wu Li-Juan, Long Lan, Sun Jia-Yi, Bu Lin-Li, Cao Jia-Lin, Luo Yao, Liu Hong-Jing, Wu Yong, Meng Xiao
Department of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Innovative institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
J Food Biochem. 2021 Jul 18:e13781. doi: 10.1111/jfbc.13781.
This study aimed to evaluate the antioxidant effect of Lactobacillus plantarum SCS2 (L. plantarum SCS2). After 1 week of acclimation, 120 male KM mice were divided into normal group (NG), model group (MG), solvent control group (KG), and different test groups (TG1, TG2, TG3) (n = 20/group) randomly. In the second week, except NG mice, other mice were given 0.2 ml 50 mg/kg (body weight) streptozocin (STZ) through intraperitoneal injection for 5 days. After successful modeling, NG and MG mice were fed normally, KG mice was given 0.5 ml 0.1 mol/L phosphate buffer saline (PBS) per day, TG1, TG2, and TG3 mice were given 0.5 ml suspension, intracellular content and heat-killed intracellular content of L. plantarum SCS2 per day for 9 weeks. Body weight and blood glucose were observed and recorded during intragastric administration. Glucose tolerance levels were measured at the twelfth week, then mice were sacrificed and the serum was collected to measure insulin (INS), glycosylated hemoglobin (HbA1c), malondialdehyde (MDA), reactive oxygen species (ROS) and antioxidant enzymes. The results showed that the reduction of weight loss in TG1 and TG2 mice was observed, which was consistent with the blood glucose. At the same time, the INS level of TG1, TG2, and TG3 mice were increased and the HbA1c levels were decreased. Otherwise, the MDA and ROS content in the serum of TG1, TG2, and TG3 mice were decreased and the level of antioxidant enzymes was increased. Interestingly, the activity and content of antioxidant enzymes in TG2 group was the highest in the three test groups. PRACTICAL APPLICATIONS: The results of this study showed that L. plantarum SCS2 could effectively reduce blood glucose, relieve weight loss, improve INS deficiency, and also improve oxidative stress by increasing the activity of antioxidant enzymes. The findings suggest that L. plantarum SCS2 could improve diabetes-related symptoms by alleviating oxidative stress. In the future, people could promote the application of lactic acid bacteria (LAB) which is found in traditional foods with the ability of improving oxidative damage in food nutrition and related fields, so as to guide residents to form good dietary habits, and effectively prevent type 2 diabetes. Meanwhile, it also can enhance the edible value of traditional foods.
本研究旨在评估植物乳杆菌SCS2（L. plantarum SCS2）的抗氧化作用。适应1周后，将120只雄性KM小鼠随机分为正常组（NG）、模型组（MG）、溶剂对照组（KG）和不同试验组（TG1、TG2、TG3）（每组n = 20）。在第二周，除NG组小鼠外，其他小鼠腹腔注射0.2 ml 50 mg/kg（体重）链脲佐菌素（STZ），连续注射5天。成功建模后，NG组和MG组小鼠正常饲养，KG组小鼠每天给予0.5 ml 0.1 mol/L磷酸盐缓冲盐水（PBS），TG1、TG2和TG3组小鼠每天给予0.5 ml植物乳杆菌SCS2的活菌悬液、死菌悬液，持续9周。灌胃给药期间观察并记录体重和血糖。在第12周测量葡萄糖耐量水平，然后处死小鼠并收集血清，以检测胰岛素（INS）、糖化血红蛋白（HbA1c）、丙二醛（MDA）、活性氧（ROS）和抗氧化酶。结果显示，观察到TG1和TG2组小鼠体重减轻减少，这与血糖情况一致。同时，TG1、TG2和TG3组小鼠的INS水平升高，HbA1c水平降低。此外，TG1、TG2和TG3组小鼠血清中的MDA和ROS含量降低，抗氧化酶水平升高。有趣的是，TG2组抗氧化酶的活性和含量在三个试验组中最高。实际应用：本研究结果表明，植物乳杆菌SCS2可有效降低血糖、减轻体重减轻、改善INS缺乏，还可通过提高抗氧化酶活性改善氧化应激。研究结果表明，植物乳杆菌SCS2可通过减轻氧化应激改善糖尿病相关症状。未来，人们可以在食品营养等相关领域推广具有改善氧化损伤能力的传统食品中发现的乳酸菌（LAB）的应用，以引导居民形成良好的饮食习惯，并有效预防2型糖尿病。同时，它还可以提高传统食品的食用价值。
