人乳头瘤病毒感染和肿瘤微环境与口咽癌患者的微生物群有关——初步研究。
Human papillomavirus infection and tumor microenvironment are associated with the microbiota in patients with oropharyngeal cancers-pilot study.
机构信息
Department of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia.
The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane, Queensland, Australia.
出版信息
Head Neck. 2021 Nov;43(11):3324-3330. doi: 10.1002/hed.26821. Epub 2021 Jul 19.
BACKGROUND
Previous microbiome studies of oropharyngeal cancer have shown that there are differences in the oral microbiota between human papillomavirus (HPV)-positive and HPV-negative patients.
METHODS
We collected saliva, normal tissue, and tumor biopsies from 13 patients with oropharyngeal cancer (eight HPV-positive, five HPV-negative). We obtained basic clinical data from each patient. Extracted DNA was 16S rRNA gene sequenced. Analysis was based on HPV status and sample site using univariate, multivariate, and mixed effect regression methods.
RESULTS
Multivariate analysis methods separated samples based on HPV status (Adonis, p < 0.001). Comparison of patients showed that there were significant changes in microbial richness across all sites based on HPV status (linear mixed effects regression, p = 0.0002).
CONCLUSIONS
We found significant differences in overall microbial community and bacterial richness between oropharyngeal patients based on HPV status. Our results suggest that there are significant differences in the microbiome in patients with oropharyngeal cancer based on HPV status.
背景
先前对口咽癌的微生物组研究表明，HPV 阳性和 HPV 阴性患者的口腔微生物群存在差异。
方法
我们收集了 13 名口咽癌患者（8 名 HPV 阳性，5 名 HPV 阴性）的唾液、正常组织和肿瘤活检样本。从每位患者获得基本临床数据。提取的 DNA 进行 16S rRNA 基因测序。使用单变量、多变量和混合效应回归方法，根据 HPV 状态和样本部位进行分析。
结果
多变量分析方法根据 HPV 状态（Adonis，p < 0.001）对样本进行了分离。对患者的比较表明，基于 HPV 状态，所有部位的微生物丰富度都有显著变化（线性混合效应回归，p = 0.0002）。
结论
我们发现 HPV 状态对口咽癌患者的整体微生物群落和细菌丰富度有显著差异。我们的结果表明，HPV 状态对口咽癌患者的微生物组存在显著差异。
Zotero 插件