Section of Epidemiology and Population Sciences, Department of Medicine, Baylor College of Medicine, Houston, TX, USA.
Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Oral Oncol. 2023 Jul;142:106401. doi: 10.1016/j.oraloncology.2023.106401. Epub 2023 May 11.
To characterize the oral microbiota among middle-aged men and identify differences between men with a prevalent oral high-risk (oncogenic) HPV infection and those without.
This was a case-control study nested within a prospective screening study for HPV-related cancers among middle-aged men. 16S rRNA sequencing was used to characterize the oral microbiota and the cobas HPV Test was used to detect presence of oral high-risk HPV types. We determined the overall composition of the oral microbiota and assessed differences in relative abundance of bacterial taxa as well as alpha and beta diversity among men with a prevalent oral high-risk HPV infection compared to men who were HPV-negative.
Among 13 high-risk HPV-positive and 30 HPV-negative men, we found significant differences in beta diversity but not alpha diversity. Fretibacterium, F0058, Kingella, Treponema, and Prevotella were more abundant among the high-risk HPV-positive men while Neisseria and Lactobacillus were more abundant among the HPV-negative men.
This study adds to the evidence that the oral microbiota varies according to oral HPV infection status and may be associated with the natural history of oral HPV infection.
描述中年男性口腔微生物群，并确定存在常见口腔高危（致癌）HPV 感染的男性与不存在感染的男性之间的差异。
这是一项病例对照研究，嵌套在一项针对中年男性 HPV 相关癌症的前瞻性筛查研究中。16S rRNA 测序用于描述口腔微生物群，而 cobas HPV 检测用于检测口腔高危 HPV 类型的存在。我们确定了口腔微生物群的总体组成，并评估了存在常见口腔高危 HPV 感染的男性与 HPV 阴性男性之间细菌分类群相对丰度以及 alpha 和 beta 多样性的差异。
在 13 名高危 HPV 阳性和 30 名 HPV 阴性男性中，我们发现了 beta 多样性的显著差异，但 alpha 多样性没有差异。Fretibacterium、F0058、Kingella、Treponema 和 Prevotella 在高危 HPV 阳性男性中更为丰富，而 Neisseria 和 Lactobacillus 在 HPV 阴性男性中更为丰富。
这项研究增加了证据表明，口腔微生物群根据口腔 HPV 感染状态而变化，并且可能与口腔 HPV 感染的自然史有关。
