海鱼丽鲻和黄带鲱的轴上肌中丙酮酸激酶的动力学特性

Kinetic properties of pyruvate kinase from the epaxial muscle of the marine fishes Mugil lisa and Chaetoditerus faber.

作者信息

Ocampos D, Rosa C D, Rodrigues E, Rosa R

机构信息

Departamento de Criação de Ruminantes e Alimentação Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil.

出版信息

Comp Biochem Physiol B. 1987;88(2):625-30. doi: 10.1016/0305-0491(87)90354-3.

DOI:10.1016/0305-0491(87)90354-3

PMID:3427907

Abstract

Kinetic studies were carried out on the reaction catalyzed by pyruvate kinase (ATP:pyruvate phosphotransferase, E.C. 2.7.1.40) purified from white striated (epaxial) muscle of two marine fish Mugil lisa (Brazilian mullet) and Chaetoditerus faber (harvest fish). This included the establishment of kinetic parameters. Attention was given to the effect of fructose 1,6-bisphosphate (Fru-P2) on PK activity. Effects of ATP, alanine and the divalent ions, Mg2+, Mn2+, Cu2+, Be2+ and Co2+, on the fish muscle enzyme were also studied.

摘要

对从两种海鱼巴西鲻鱼（Mugil lisa）和细纹油鲱（Chaetoditerus faber）的白色横纹（轴上肌）肌肉中纯化得到的丙酮酸激酶（ATP：丙酮酸磷酸转移酶，E.C. 2.7.1.40）所催化的反应进行了动力学研究。这包括动力学参数的确定。重点关注了1,6-二磷酸果糖（Fru-P2）对丙酮酸激酶活性的影响。还研究了ATP、丙氨酸以及二价离子Mg2+、Mn2+、Cu2+、Be2+和Co2+对鱼肌肉酶的影响。