Drouin R, Rémy-Prince S, Richer C L, Montplaisir S
Department of Anatomy, Faculty of Medicine, Université de Montréal, Québec, Canada.
Cytobios. 1987;52(210-211):135-52.
The in vitro effects of four protein supplements, foetal calf serum (FCS), gammaglobulin-depleted FCS (FCS-A), human serum albumin (HSA) and autologous serum (AS), and of four antibiotics on lymphocyte blast transformation and on lymphocyte proliferation for cytogenetic studies, were evaluated. 3H-thymidine incorporation was measured in the lymphocytes obtained from sixteen healthy adults and stimulated with phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), PWM + PHA and PWM + Con A. The most efficient protein supplement was AS, although great variability was observed between subjects. HSA came second, while FCS and FCS-A were the least effective. The presence of immunoglobulins did not modify mitogen-induced lymphocyte responses. In unsupplemented media, peak responses to PHA and Con A occurred at lower mitogen concentrations. Furthermore, the addition of the antibiotics tested did not impede blast transformation whether or not a protein supplement was present. This work also showed that for chromosome analysis, protein supplements are necessary for optimum whole-blood lymphocyte cultures and that HSA can replace FCS.
评估了四种蛋白质补充剂（胎牛血清（FCS）、γ球蛋白缺失的FCS（FCS-A）、人血清白蛋白（HSA）和自体血清（AS））以及四种抗生素对淋巴细胞增殖转化和用于细胞遗传学研究的淋巴细胞增殖的体外作用。在来自16名健康成年人的淋巴细胞中测量3H-胸腺嘧啶核苷掺入量，并用植物血凝素（PHA）、刀豆蛋白A（Con A）、商陆有丝分裂原（PWM）、PWM + PHA和PWM + Con A进行刺激。最有效的蛋白质补充剂是AS，尽管个体之间存在很大差异。HSA位居第二，而FCS和FCS-A效果最差。免疫球蛋白的存在并未改变有丝分裂原诱导的淋巴细胞反应。在未补充培养基中，对PHA和Con A的峰值反应出现在较低的有丝分裂原浓度下。此外，无论是否存在蛋白质补充剂，所测试抗生素的添加均未阻碍增殖转化。这项工作还表明，对于染色体分析，蛋白质补充剂对于优化全血淋巴细胞培养是必要的，并且HSA可以替代FCS。
