Department of Ecological Symbiotic Science, Graduate school of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Department of Molecular Microbiology, Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Microbiology (Reading). 2021 Jul;167(7). doi: 10.1099/mic.0.001071.
Siderophores are produced by several bacteria that utilise iron in various environments. Elucidating the structure of a specific siderophore may have valuable applications in drug development. , a Gram-negative bacterium that inhabits a wide range of environments and can cause pneumonia, produces siderophores. However, the structure was unknown, and therefore, in this study, we aimed to elucidate it. We purified siderophores from cultures of K279a using preparative reversed-phase HPLC. The structure was analysed through LC-MS and H and C NMR. The results demonstrated that K279a produces 2,3-dihydroxybenzoylserine (DHBS), a monomer unit of enterobactin. We suggested the uptake of Iron(III) by the DHBS complex. DHBS production by K279a could be attributed to an incomplete enterobactin pathway. Drugs targeting DHBS synthesis could prevent infection.
铁载体由几种在各种环境中利用铁的细菌产生。阐明特定铁载体的结构可能在药物开发中有重要的应用。K279a 是一种革兰氏阴性菌，栖息于广泛的环境中，可引起肺炎，产生铁载体。然而，其结构尚不清楚，因此，在本研究中，我们旨在阐明其结构。我们使用制备性反相 HPLC 从 K279a 的培养物中纯化铁载体。通过 LC-MS 和 H 和 C NMR 分析其结构。结果表明，K279a 产生 2,3-二羟基苯甲酰丝氨酸（DHBS），这是 enterobactin 的单体单元。我们提出了 DHBS 配合物摄取三价铁。K279a 产生 DHBS 可能是由于 enterobactin 途径不完整。针对 DHBS 合成的药物可能可以预防 感染。
