García Carlos A, Passerini De Rossi Beatriz, Alcaraz Eliana, Vay Carlos, Franco Mirta
Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 (1113) Ciudad Autónoma de Buenos Aires, Argentina.
Rev Argent Microbiol. 2012 Jul-Sep;44(3):150-4.
Stenotrophomonas maltophilia is an emerging nosocomial pathogen. Despite the broad spectrum of syndromes associated with S. maltophilia infections, little is known about its virulence factors, including siderophore production. The aims of this work were to detect S. maltophilia siderophores and to determine their chemical nature. We studied 31 S. maltophilia isolates from device-associated infections, recovered over the period 2006-2011 at Hospital de Clínicas José de San Martín, Buenos Aires, Argentina, and the strain K279a, whose genome has been fully sequenced. The production of siderophores was screened by the chrome azurol S (CAS) agar assay, previously modified to detect siderophores in this species. When grown on modified CAS agar plates, all the clinical isolates and K279a were CAS-positive for siderophore production. In order to determine the chemical nature of siderophores, the Csáky (hydroxamate-type) and Arnow (catechol-type) assays were used. All S. maltophilia isolates produced catechol-type siderophores, but hydroxamate-type siderophores were not detected.
嗜麦芽窄食单胞菌是一种新出现的医院病原体。尽管嗜麦芽窄食单胞菌感染相关的综合征范围广泛，但对其毒力因子，包括铁载体的产生了解甚少。这项工作的目的是检测嗜麦芽窄食单胞菌的铁载体并确定其化学性质。我们研究了2006年至2011年期间在阿根廷布宜诺斯艾利斯何塞·德·圣马丁临床医院从与器械相关感染中分离出的31株嗜麦芽窄食单胞菌，以及已完成全基因组测序的K279a菌株。通过铬天青S（CAS）琼脂试验筛选铁载体的产生，该试验先前已进行改良以检测该物种中的铁载体。当在改良的CAS琼脂平板上生长时，所有临床分离株和K279a的铁载体产生均为CAS阳性。为了确定铁载体的化学性质，使用了恰基（异羟肟酸型）和阿诺（儿茶酚型）试验。所有嗜麦芽窄食单胞菌分离株均产生儿茶酚型铁载体，但未检测到异羟肟酸型铁载体。
