State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China; School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi 214122, PR China.
Int J Biol Macromol. 2022 May 31;208:669-677. doi: 10.1016/j.ijbiomac.2022.03.140. Epub 2022 Mar 26.
The development of new antimicrobial agents is important to combat infections caused by pathogenic bacteria. Herein, Hydroxypropyl chitosan (HPCS), a hydrophilic modified product of chitosan (CS), was employed as a carrier of the photosensitizer chlorin e6 (Ce6) through an amide bond to obtain the products (HPCS-Ce6 conjugates) with a degree of substitution (DS) ranging from 2.95% to 5.25%. The UV-vis absorption spectra and H NMR spectra confirmed the successful synthesis of the products. The products have a better and more stable reactive oxygen species (ROS) generation capacity and higher bacterial affinity than Ce6. At a very low dose (1.8 μg/mL), the highest DS product (HPCS-Ce6-3) can effectively kill Staphylococcus aureus (S. aureus) under 660 nm irradiation. In addition, the HPCS-Ce6 conjugates showed high biocompatibility in the CCK-8 test. The HPCS-Ce6 conjugates could be a photodynamic antibacterial agent with good water solubility, high biocompatibility, and antibacterial activity.
开发新的抗菌剂对于对抗由病原菌引起的感染非常重要。在此，通过酰胺键将壳聚糖（CS）的亲水改性产物羟丙基壳聚糖（HPCS）用作光敏剂氯代乙酮（Ce6）的载体，获得取代度（DS）范围为 2.95%至 5.25%的产物（HPCS-Ce6 缀合物）。紫外-可见吸收光谱和 H NMR 光谱证实了产物的成功合成。与 Ce6 相比，产物具有更好、更稳定的活性氧（ROS）生成能力和更高的细菌亲和力。在非常低的剂量（1.8μg/mL）下，最高 DS 产物（HPCS-Ce6-3）在 660nm 照射下可以有效杀死金黄色葡萄球菌（S. aureus）。此外，在 CCK-8 试验中，HPCS-Ce6 缀合物表现出高的生物相容性。HPCS-Ce6 缀合物可以成为一种具有良好水溶性、高生物相容性和抗菌活性的光动力抗菌剂。
