Roberts V J, Gorenstein C
Department of Pharmacology, University of California, Irvine.
Dev Neurosci. 1987;9(4):255-64. doi: 10.1159/000111628.
We have previously observed that lysosomes redistribute from their normal location in neuronal cell bodies to the dendrites following an intracerebroventricular injection of an antimitotic such as colchicine, vinblastine or vincristine. In the present study, we have followed the developmental distribution of lysosomes in the brains of untreated rats, using a lysosomal marker enzyme, dipeptidylaminopeptidase II. A relatively high concentration of neuronal lysosomes was found in the dendrites of olfactory bulb mitral cell neurons and in hippocampal granule and pyramidal cell neurons from postnatal day 1 (P1) to P8. As the animals matured, the pattern of lysosomal enzyme distribution was reversed. Lysosomes became progressively less concentrated in the dendrites and more concentrated in neuronal cell bodies. In cerebellar Purkinje cells, lysosomes were only found in the cell bodies during the first week after birth. Between P9 and P19, lysosomes appeared in the dendrites of these neurons and, with maturity, progressively disappeared from the dendrites and were concentrated mainly in cell bodies. The presence of lysosomes in the dendrites of developing animals suggests that the transport of lysosomes to the dendrites, induced by microtubule poisons, mimics a physiological process which is normally present during development.
我们之前观察到，在脑室内注射秋水仙碱、长春花碱或长春新碱等抗有丝分裂药物后，溶酶体会从其在神经元细胞体中的正常位置重新分布到树突中。在本研究中，我们使用溶酶体标记酶二肽基氨基肽酶II，追踪了未处理大鼠大脑中溶酶体的发育分布情况。在出生后第1天（P1）至P8，嗅球二尖瓣细胞神经元以及海马颗粒细胞和锥体细胞神经元的树突中发现了相对较高浓度的神经元溶酶体。随着动物成熟，溶酶体酶的分布模式发生了逆转。溶酶体在树突中的浓度逐渐降低，而在神经元细胞体中的浓度逐渐升高。在小脑浦肯野细胞中，出生后的第一周内仅在细胞体中发现溶酶体。在P9至P19期间，这些神经元的树突中出现了溶酶体，随着成熟，溶酶体逐渐从树突中消失，主要集中在细胞体中。发育中的动物树突中存在溶酶体表明，微管毒物诱导的溶酶体向树突的运输模拟了发育过程中正常存在的生理过程。
