Gorenstein C, Ribak C E
J Neurosci. 1985 Aug;5(8):2018-27. doi: 10.1523/JNEUROSCI.05-08-02018.1985.
The effect of colchicine injections on the ultrastructural localization of dipeptidyl peptidase II (Dpp II) was studied in the mitral cells of the rat olfactory bulb. In control animals, electron-dense reaction product representing Dpp II activity was observed in lysosomes, lipofuscin granules, short cisternae located close to the granular endoplasmic reticulum, and dense granules. Lysosomes and lipofuscin granules were the most intensely stained organelles. Dpp II-containing organelles were localized mainly to the cell body and were randomly distributed in the perikaryal cytoplasm. Twenty-four hours after a 100-micrograms intracerebroventricular colchicine injection, the distribution of Dpp II-containing organelles was drastically altered. Short cisternae and dense granules containing Dpp II reaction product were noticeably absent in these preparations. Lysosomes and lipofuscin granules were depleted from the perikaryal cytoplasm and were concentrated in dendrites. Lysosomes were observed to extend for considerable distances in dendrites where they acquired elongated and dumbbell shapes. The shapes of some of these labeled lysosomes gave the impression that they were actively being "pulled" into the dendrites. These results indicate that microtubules sequester lysosomes to the perikaryal cytoplasm and suggest the presence of a novel transport system responsible for the movement of lysosomes from the cell body to the dendrites.
研究了秋水仙碱注射对大鼠嗅球二尖瓣细胞中二肽基肽酶II（Dpp II）超微结构定位的影响。在对照动物中，在溶酶体、脂褐素颗粒、靠近颗粒内质网的短池以及致密颗粒中观察到代表Dpp II活性的电子致密反应产物。溶酶体和脂褐素颗粒是染色最强烈的细胞器。含有Dpp II的细胞器主要定位于细胞体，并随机分布在核周细胞质中。脑室内注射100微克秋水仙碱24小时后，含有Dpp II的细胞器的分布发生了显著变化。在这些制剂中明显没有含有Dpp II反应产物的短池和致密颗粒。溶酶体和脂褐素颗粒从核周细胞质中耗尽，并集中在树突中。观察到溶酶体在树突中延伸相当长的距离，在那里它们呈现出细长和哑铃状。其中一些标记的溶酶体的形状给人的印象是它们正被积极地“拉”入树突中。这些结果表明微管将溶酶体隔离在核周细胞质中，并提示存在一种负责溶酶体从细胞体向树突移动的新型运输系统。
