Department of Veterinary Science, Università di Parma, Parma, Italy.
Department of Medicine and Surgery, Università di Milano-Bicocca, Monza, Italy.
Sci Rep. 2022 May 24;12(1):8742. doi: 10.1038/s41598-022-12738-9.
Single cell classification is elucidating homeostasis and pathology in tissues and whole organs. We applied in situ spatial proteomics by multiplex antibody staining to routinely processed mouse lung, healthy and during a fibrosis model. With a limited validated antibody panel (24) we classify the normal constituents (alveolar type I and II, bronchial epithelia, endothelial, muscular, stromal and hematopoietic cells) and by quantitative measurements, we show the progress of lung fibrosis over a 4 weeks course, the changing landscape and the cell-specific quantitative variation of a multidrug transporter. An early decline in AT2 alveolar cells and a progressive increase in stromal cells seems at the core of the fibrotic process.
单细胞分类阐明了组织和整个器官的动态平衡和病理学。我们通过多重抗体染色的原位空间蛋白质组学方法，对常规处理的小鼠肺、健康肺和纤维化模型肺进行了研究。在使用有限的经验证抗体面板（24 个）的情况下，我们对正常成分（I 型和 II 型肺泡细胞、支气管上皮细胞、内皮细胞、肌肉细胞、基质细胞和造血细胞）进行了分类，并通过定量测量，显示了肺纤维化在 4 周过程中的进展、不断变化的图谱以及多药转运蛋白的细胞特异性定量变化。II 型肺泡细胞的早期减少和基质细胞的逐渐增加似乎是纤维化过程的核心。
