Li Jialin, Luan Qianqian, Han Jing, Chen Chunhua, Ren Zhonghai
State Key Laboratory of Crop Biology, Shandong Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China.
School of Biological Science and Technology, University of Jinan, Jinan 250022, China.
Phytopathology. 2022 Mar;112(3):588-594. doi: 10.1094/PHYTO-05-21-0223-R. Epub 2022 Feb 28.
Root rot caused by is one of the most common fungal diseases in cucumber (). Proanthocyanidins (PAs) are known to play important roles in inhibiting the growth of phytopathogens. In addition, CsMYB60 is a known positive regulator of flavonol and PA biosynthesis in cucumber. However, it remains unclear that whether PAs can inhibit the growth of and whether serves as a target gene for increasing resistance to phytopathogens in cucumber. In this study, we demonstrated that PAs (or their building block, catechin) could increase the resistance of cucumber seedlings to both in vitro and in vivo. The addition of catechins, or crude leaf extracts treated with different concentrations of catechins in culture medium, could significantly inhibit the hyphal growth of . On the other hand, cucumber seedlings treated with catechins showed higher resistance to than the seedlings of control group. Moreover, transgenic cucumber seedlings overexpressing , with the observed accumulation of PAs, were more resistant to than the nontransgenic siblings. Therefore, our results suggest that PAs (or catechin) can serve as a biological control agent to protect cucumber plants from the infection of . More importantly, holds great promise as a target gene to confer disease resistance during the molecular breeding in cucumber.
由[病原体名称未给出]引起的根腐病是黄瓜（[黄瓜学名未给出]）中最常见的真菌病害之一。已知原花青素（PAs）在抑制植物病原体生长方面发挥重要作用。此外，CsMYB60是黄瓜中黄酮醇和PA生物合成的已知正调控因子。然而，PAs是否能抑制[病原体名称未给出]的生长以及CsMYB60是否作为黄瓜中增强对植物病原体抗性的靶基因仍不清楚。在本研究中，我们证明PAs（或其组成成分儿茶素）在体外和体内均可提高黄瓜幼苗对[病原体名称未给出]的抗性。在培养基中添加儿茶素或用不同浓度儿茶素处理的粗叶提取物，可显著抑制[病原体名称未给出]的菌丝生长。另一方面，用儿茶素处理的黄瓜幼苗比对照组幼苗对[病原体名称未给出]表现出更高的抗性。此外，过表达[基因名称未给出]且观察到PA积累的转基因黄瓜幼苗比非转基因同胞对[病原体名称未给出]更具抗性。因此，我们的结果表明PAs（或儿茶素）可作为一种生物防治剂来保护黄瓜植株免受[病原体名称未给出]的感染。更重要的是，[基因名称未给出]在黄瓜分子育种过程中作为赋予抗病性的靶基因具有很大潜力。
