Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS Deemed to be University, Vile Parle (West), Mumbai, India.
Curr Microbiol. 2021 Sep;78(9):3372-3380. doi: 10.1007/s00284-021-02613-7. Epub 2021 Jul 20.
A temperature downshift results in stabilized secondary structure formation in mRNA that halts translation to which Escherichia coli responds by synthesizing a set of proteins termed as cold shock proteins (Csps). To cope with the sudden temperature drop, gene expression patterns are reprogrammed to induce Csps at the cost of other proteins. Out of the nine homologous proteins in the CspA family, CspA, CspB, CspG, and CspI have major roles in protecting the cell under a cold shock. Additionally, a subset of Csps has conferred the organism an ability to adapt to various stresses along the lines of nutrient deprivation, oxidative, heat, acid, and antibiotic stresses. Stressors like C group translational inhibitors stall the translational apparatus and produce a response similar to that observed under a temperature downshift. Conditions set by the antibiotic therefore elicit a cold shock response and induce the major Csps, thereby pointing out to a common mechanism existing between the two. In the current review, we briefly describe the induction of E. coli Csps under an antibiotic stress acquired from data published previously and help establish the role of Csps in protecting the cell against the inducing agents and as a participant in the organisms' complex stress response network.
温度下降会导致 mRNA 中稳定的二级结构形成，从而阻止翻译，大肠杆菌对此的反应是合成一组称为冷休克蛋白 (Csps) 的蛋白质。为了应对突然的温度下降，基因表达模式被重新编程，以诱导 Csps 的表达，而牺牲其他蛋白质。在 CspA 家族的 9 种同源蛋白中，CspA、CspB、CspG 和 CspI 在冷休克下保护细胞方面发挥着重要作用。此外，一组 Csps 赋予了生物体适应各种应激的能力，包括营养剥夺、氧化、热、酸和抗生素应激。像 C 族翻译抑制剂这样的应激物会使翻译装置停滞不前，并产生类似于温度下降时观察到的反应。因此，抗生素设定的条件会引发冷休克反应并诱导主要的 Csps，从而指出这两种情况之间存在共同的机制。在当前的综述中，我们简要描述了先前发表的数据中获得的抗生素应激下大肠杆菌 Csps 的诱导，并帮助确定了 Csps 在保护细胞免受诱导剂的侵害以及作为生物体复杂应激反应网络的参与者方面的作用。
