Yi Chuan-An, Jiang Yu-Hong, Wang Ye, Li Yu-Xian, Cai Shi-Chang, Wu Xiu-Yu, Hu Xiang-Shang, Wan Xing-Guang
Medical Morphology Experimental Center, Hunan University of Medicine, Hunan, People's Republic of China.
Key Laboratory of Dong Medical Research Hunan Province, Hunan, People's Republic of China.
Neuropsychiatr Dis Treat. 2021 Jul 12;17:2257-2267. doi: 10.2147/NDT.S314162. eCollection 2021.
The study aimed to explore the effects of treatment with black bamboo rhizome extracts on learning and memory and determine the underlying mechanisms in rats with cerebral ischaemia-reperfusion injury.
Sprague-Dawley rats were randomly divided into the following four groups: control, middle cerebral artery occlusion (MCAO), low-dose drug, and high-dose drug groups. Rats underwent MCAO using a suture method before drug treatment. Then, neurological impairment was assessed using the Longa scoring method, and triphenyl tetrazolium chloride staining was used to analyse the cerebral infarction area. The Elliott formula was used to calculate water content in the brain tissue. A Morris water maze (MWM) was used to assess changes in learning and memory abilities, and Western blotting was used to detect cyclic adenosine phosphate response element-binding protein (CREB) and brain-derived neurotrophic factor (BDNF) expression in the hippocampus of MCAO rats.
After treatment with black bamboo rhizome extracts, the neurological dysfunction score was lower in the drug groups than in the MCAO group, and a significant difference was observed between the high-dose drug and MCAO groups (P<0.05). Additionally, the cerebral infarction area was significantly smaller in the drug groups than in the MCAO group (P<0.01), and the effect was more obvious in the high-dose drug group than in the low-dose drug group. There was also a significant difference in water content between the high-dose drug and MCAO groups, and cerebral oedema was significantly reduced in the high-dose drug group (P<0.05). In the MWM, the incubation period was significantly reduced, the number of platform crossings was significantly increased, and the search time was prolonged in the drug groups compared with those in the MCAO group (P<0.05). Moreover, the expression of BDNF and CREB was significantly increased in the drug groups compared to that in the MCAO group, and the increase was more obvious in the high-dose group than in the low-dose group (P<0.05).
Black bamboo rhizome extracts significantly improved cognitive dysfunction, reduced cerebral oedema, decreased the cerebral infarction area, and improved the neurological function score and learning and memory abilities in rats with cerebral ischaemia-reperfusion injury.
本研究旨在探讨黑竹根茎提取物治疗对脑缺血再灌注损伤大鼠学习记忆的影响，并确定其潜在机制。
将Sprague-Dawley大鼠随机分为以下四组：对照组、大脑中动脉闭塞（MCAO）组、低剂量药物组和高剂量药物组。在药物治疗前，采用缝线法对大鼠进行MCAO。然后，使用Longa评分法评估神经功能缺损，并用氯化三苯基四氮唑染色分析脑梗死面积。采用Elliott公式计算脑组织含水量。使用Morris水迷宫（MWM）评估学习记忆能力的变化，并用蛋白质免疫印迹法检测MCAO大鼠海马中磷酸环腺苷反应元件结合蛋白（CREB）和脑源性神经营养因子（BDNF）的表达。
用黑竹根茎提取物治疗后，药物组的神经功能障碍评分低于MCAO组，高剂量药物组与MCAO组之间存在显著差异（P<0.05）。此外，药物组的脑梗死面积明显小于MCAO组（P<0.01），高剂量药物组的效果比低剂量药物组更明显。高剂量药物组与MCAO组的含水量也存在显著差异，高剂量药物组的脑水肿明显减轻（P<0.05）。在MWM中，与MCAO组相比，药物组的潜伏期明显缩短，穿越平台的次数明显增加，搜索时间延长（P<0.05）。此外，与MCAO组相比，药物组中BDNF和CREB的表达明显增加，高剂量组的增加比低剂量组更明显（P<0.05）。
黑竹根茎提取物可显著改善脑缺血再灌注损伤大鼠的认知功能障碍，减轻脑水肿，减小脑梗死面积，并改善神经功能评分及学习记忆能力。
