Capy P, Rouault J
Laboratoire de Biologie et Génétique Evolutives, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Genetics. 1987 Dec;117(4):795-801. doi: 10.1093/genetics/117.4.795.
The number of alleles present in a natural population of unknown structure is estimated using a sequential sampling procedure applied to isofemale lines. Two questions are raised: how many individuals per isofemale line must be assayed and how many isofemale lines must be sampled to get an adequate sample to estimate the number of alleles, at a given risk, of the natural population? On the one hand, we show that when wild females are inseminated once, only two individuals per line are required. On the other hand, the number of isofemale lines that must be sampled depends on the risk chosen of losing an allele, on the number of alleles present in the population and on their drawing probabilities. When the population structure is known, an accurate answer can be provided. For an unknown population structure, one general sequential sampling previously described by J. Rouault and P. Capy is proposed to estimate the number of alleles in the population from data on isofemale lines.
利用应用于同雌系的序贯抽样程序来估计结构未知的自然种群中存在的等位基因数量。由此引出两个问题：每个同雌系必须检测多少个体，以及必须抽样多少个同雌系才能获得足够的样本，以便在给定风险下估计自然种群的等位基因数量？一方面，我们表明，当野生雌性只进行一次授精时，每条系只需两个个体。另一方面，必须抽样的同雌系数量取决于所选择的丢失一个等位基因的风险、种群中存在的等位基因数量及其抽取概率。当种群结构已知时，可以给出准确答案。对于未知的种群结构，提出了一种先前由J.鲁奥尔特和P.卡皮描述的通用序贯抽样方法，以便根据同雌系的数据估计种群中的等位基因数量。
