Aoki-Utsubo Chie, Chen Ming, Hotta Hak
Department of International Health, Graduate School of Health Sciences, Kobe University, Kobe, Japan.
Department of Vaccine and Drug Development, Graduate School of Health Sciences, Kobe University, Kobe, Japan.
Bio Protoc. 2018 May 20;8(10):e2855. doi: 10.21769/BioProtoc.2855.
In a narrow definition, virucidal activity represents the activity by which to interact with and physically disrupt viral particles. In a broad definition, it includes the activity by which to functionally inhibit (neutralize) viral infectivity without apparent morphological alterations of the viral particles. The viral infectivity can be measured in cell culture system by means of plaque assay, infectious focus assay, 50% tissue culture infectious dose (TCID) assay, . Morphologically, disruption of viral particles can be demonstrated by negative staining electron microscopic analysis of viral particles. In this article, we describe methods to assess virucidal activity in a broad definition.
狭义地说，杀病毒活性是指与病毒颗粒相互作用并使其物理性破坏的活性。广义地说，它包括在病毒颗粒无明显形态改变的情况下功能性抑制（中和）病毒感染性的活性。病毒感染性可在细胞培养系统中通过噬斑测定、感染灶测定、50%组织培养感染剂量（TCID）测定等来测量。在形态学上，病毒颗粒的破坏可通过对病毒颗粒进行负染色电子显微镜分析来证明。在本文中，我们描述了评估广义杀病毒活性的方法。
