Shimizu K, Mukaigawa J, Oguro M, Ono Y, Nakajima K, Kida H
Vaccine. 1985 Sep;3(3 Suppl):207-10. doi: 10.1016/0264-410x(85)90107-0.
An investigation was made of inhibition of transcriptase activity of influenza viruses in vitro by binding of antibody to the surface of the virion. Eight monoclonal antibodies which were directed against at least four non-overlapping antigenic regions of the haemagglutinin protein of A/Aichi/68 virus were tested for inhibitory effect. One of the antibodies directed against the B antigenic site, 22/1, inhibited transcriptase activity, while the other seven antibodies did not. Antibody from a hyperimmune rabbit serum to A/Udorn/72 (H3N2) virions inhibited the transcriptase activity of A/Udorn/72 and A/Aichi/68 (H3N2) viruses but not that of A/WSN/33 (H1N1). The antibody did not cause irreversible inactivation of the transcriptase since full activity was recovered by isolating ribonucleoprotein (RNP) cores from the inhibited virions using NP-40 treatment and subsequent centrifugation in a caesium sulphate density gradient. The antibody did not inhibit transcriptase activity of isolated RNP cores. The virion transcriptase activity was not inhibited by addition of the antiserum after the detergent treatment which is necessary for the activation of the transcriptase activity in vitro. These results suggest that the antibody blocks the activation process of the transcriptase by detergent treatment.
通过抗体与病毒粒子表面结合对流感病毒转录酶活性的体外抑制作用进行了研究。测试了针对A/爱知/68病毒血凝素蛋白至少四个不重叠抗原区域的八种单克隆抗体的抑制作用。其中一种针对B抗原位点的抗体22/1抑制了转录酶活性，而其他七种抗体则没有。来自对A/乌冬/72（H3N2）病毒粒子的超免疫兔血清的抗体抑制了A/乌冬/72和A/爱知/68（H3N2）病毒的转录酶活性，但不抑制A/WSN/33（H1N1）病毒的转录酶活性。该抗体不会导致转录酶的不可逆失活，因为通过使用NP-40处理从受抑制的病毒粒子中分离核糖核蛋白（RNP）核心并随后在硫酸铯密度梯度中离心可恢复全部活性。该抗体不抑制分离的RNP核心的转录酶活性。在体外激活转录酶活性所需的去污剂处理后加入抗血清不会抑制病毒粒子的转录酶活性。这些结果表明，抗体通过去污剂处理阻断了转录酶的激活过程。
