从单细胞绿藻中提取RNA
RNA Purification from the Unicellular Green Alga, .
作者信息
Gallaher Sean D, Roth Melissa S
机构信息
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.
Howard Hughes Medical Institute, Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
出版信息
Bio Protoc. 2018 Apr 5;8(7):e2792. doi: 10.21769/BioProtoc.2793.
is a unicellular green alga that is an emerging model species for studies in fields such as biofuel production, ketocarotenoid biosynthesis and metabolism. The recent availability of a high-quality genome assembly facilitates systems-level analysis, such as RNA-Seq. However, cells of this alga have a tough cell wall, which is a challenge for RNA purification. This protocol was designed specifically to breach the cell wall and isolate high-quality RNA suitable for RNA-Seq studies.
是一种单细胞绿藻，是生物燃料生产、酮类胡萝卜素生物合成和代谢等领域研究中新兴的模式物种。最近高质量基因组组装的可得性促进了系统水平的分析，如RNA测序。然而，这种藻类的细胞有一层坚韧的细胞壁，这对RNA纯化来说是一个挑战。本方案专门设计用于突破细胞壁并分离适合RNA测序研究的高质量RNA。
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