Vu Thi Hao, Hong Yeojin, Truong Anh Duc, Lee Jiae, Lee Sooyeon, Song Ki-Duk, Cha Jihye, Dang Hoang Vu, Tran Ha Thi Thanh, Lillehoj Hyun S, Hong Yeong Ho
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Department of Biochemistry and Immunology, National Institute of Veterinary Research, Hanoi 100000, Vietnam.
Anim Biosci. 2022 Mar;35(3):367-376. doi: 10.5713/ab.21.0163. Epub 2021 Jun 24.
The highly pathogenic avian influenza virus (HPAIV) is a threat to the poultry industry as well as the economy and remains a potential source of pandemic infection in humans. Antiviral genes are considered a potential factor for HPAIV resistance. Therefore, in this study, we investigated gene expression related to cytokine-cytokine receptor interactions by comparing resistant and susceptible Ri chicken lines for avian influenza virus infection.
Ri chickens of resistant (Mx/A; BF2/B21) and susceptible (Mx/G; BF2/B13) lines were selected by genotyping the Mx dynamin like GTPase (Mx) and major histocompatibility complex class I antigen BF2 genes. These chickens were then infected with influenza A virus subtype H5N1, and their lung tissues were collected for RNA sequencing.
In total, 972 differentially expressed genes (DEGs) were observed between resistant and susceptible Ri chickens, according to the gene ontology and Kyoto encyclopedia of genes and genomes pathways. In particular, DEGs associated with cytokine-cytokine receptor interactions were most abundant. The expression levels of cytokines (interleukin-1β [IL-1β], IL-6, IL-8, and IL-18), chemokines (C-C Motif chemokine ligand 4 [CCL4] and CCL17), interferons (IFN-γ), and IFN-stimulated genes (Mx1, CCL19, 2'-5'-oligoadenylate synthaselike, and protein kinase R) were higher in H5N1-resistant chickens than in H5N1-susceptible chickens.
Resistant chickens show stronger immune responses and antiviral activity (cytokines, chemokines, and IFN-stimulated genes) than those of susceptible chickens against HPAIV infection.
高致病性禽流感病毒（HPAIV）对家禽业和经济构成威胁，仍是人类大流行感染的潜在来源。抗病毒基因被认为是HPAIV抗性的一个潜在因素。因此，在本研究中，我们通过比较抗禽流感病毒感染的抗性和易感Ri鸡品系，研究了与细胞因子-细胞因子受体相互作用相关的基因表达。
通过对动力蛋白样GTP酶（Mx）和主要组织相容性复合体I类抗原BF2基因进行基因分型，选择抗性（Mx/A；BF2/B21）和易感（Mx/G；BF2/B13）品系的Ri鸡。然后用甲型H5N1流感病毒感染这些鸡，并收集它们的肺组织进行RNA测序。
根据基因本体论和京都基因与基因组百科全书途径，在抗性和易感Ri鸡之间总共观察到972个差异表达基因（DEG）。特别是，与细胞因子-细胞因子受体相互作用相关的DEG最为丰富。细胞因子（白细胞介素-1β[IL-1β]、IL-6、IL-8和IL-18）、趋化因子（C-C基序趋化因子配体4[CCL4]和CCL17）、干扰素（IFN-γ）以及IFN刺激基因（Mx1、CCL19、2'-5'-寡腺苷酸合成酶样蛋白和蛋白激酶R）在H5N1抗性鸡中的表达水平高于H5N1易感鸡。
与易感鸡相比，抗性鸡在抵抗HPAIV感染时表现出更强的免疫反应和抗病毒活性（细胞因子、趋化因子和IFN刺激基因）。
