Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Department of Biochemistry and Immunology, National Institute of Veterinary Research, 86 Truong Chinh, Dong Da, Hanoi 100000, Viet Nam.
Vet Immunol Immunopathol. 2022 Apr;246:110404. doi: 10.1016/j.vetimm.2022.110404. Epub 2022 Feb 24.
Influenza A/H5N1 virus is a highly pathogenic (HPAIV) and contagious zoonotic virus that can be transmitted to humans. In the present study, infection with this virus and differential gene expression analyses were carried out with genetically resistant and susceptible Ri chicken lines that are native to Vietnam. A total of 20 four-week-old Ri chickens from each line were inoculated with the highly pathogenic H5N1 avian influenza virus (5 chickens/group). On day 3 post-infection, the total tracheal RNA was sequenced. Differentially expressed genes in the influenza A pathway, including signaling pathway-related genes, were validated by reverse transcription quantitative real-time PCR (RT-qPCR). The resistant and susceptible lines showed significant differences in gene expression and multiple biological functions. Compared to expression in the susceptible line, in the resistant line, the expression of MX1, STAT1, IRF7, and TLR3 was upregulated, while that of BF1 was downregulated. Genes from the interferon (IFN) and cytokine families, which regulate the immune system, were highly expressed in the HPAIV infected resistant line. Finally, significant differences were observed in the expression of genes encoding components of the Jak-STAT and TLR signaling pathways between the two chicken lines. Collectively, our findings suggest that HPAIV-resistant and -susceptible Ri chicken lines differed in immunity upon infection. Understanding the regulation of immune pathways against HPAIV will help to better understand the mechanisms of immune regulation in chickens.
甲型 H5N1 流感病毒是一种高致病性（HPAIV）和传染性的人畜共患病病毒，可以传播给人类。在本研究中，使用源自越南的具有遗传抗性和敏感性的 Ri 鸡系进行了感染该病毒和差异基因表达分析。从每条线共获得 20 只 4 周龄的 Ri 鸡进行接种高致病性 H5N1 禽流感病毒（每组 5 只鸡）。在感染后第 3 天，对总气管 RNA 进行测序。通过逆转录定量实时 PCR（RT-qPCR）验证流感 A 途径中的差异表达基因，包括信号通路相关基因。抗性和敏感性系在基因表达和多种生物学功能上表现出显著差异。与敏感性系的表达相比，在抗性系中，MX1、STAT1、IRF7 和 TLR3 的表达上调，而 BF1 的表达下调。调节免疫系统的干扰素（IFN）和细胞因子家族的基因在 HPAIV 感染的抗性系中高度表达。最后，在两种鸡系中，Jak-STAT 和 TLR 信号通路的基因编码成分的表达存在显著差异。总之，我们的研究结果表明，HPAIV 抗性和敏感性 Ri 鸡系在感染时的免疫反应不同。了解针对 HPAIV 的免疫途径的调节将有助于更好地了解鸡的免疫调节机制。
