Qiu Shiyou, Chen Xuwei, Zhai Yushan, Cui Weijun, Ai Xuhong, Rao Shaofei, Chen Jianping, Yan Fei
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Virology, Ningbo University, Ningbo, China.
Front Microbiol. 2021 Jul 5;12:690988. doi: 10.3389/fmicb.2021.690988. eCollection 2021.
The light-harvesting chlorophyll / complex protein 3 (LHCB3) of photosystem II plays important roles distributing the excitation energy and modulating the rate of state transition and stomatal response to abscisic acid. However, the functions of LHCB3 in plant immunity have not been well investigated. Here, we show that the expression of in () was down-regulated by turnip mosaic virus (TuMV) infection. When was silenced by tobacco rattle virus-induced gene silencing, systemic infection of TuMV was inhibited. HO was over-accumulated in -silenced plants. Chemical treatment to inhibit or eliminate reactive oxygen species (ROS) impaired the resistance of the -silenced plants to TuMV infection. Co-silencing of with genes involved in ROS production compromised the resistance of plants to TuMV but co-silencing of with genes in the ROS scavenging pathway increased resistance to the virus. Transgenic plants overexpressing were more susceptible to TuMV. These results indicate that downregulation of is involved in defense against TuMV by inducing ROS production.
光系统II的捕光叶绿素/复合蛋白3（LHCB3）在分配激发能、调节状态转换速率和气孔对脱落酸的响应方面发挥着重要作用。然而，LHCB3在植物免疫中的功能尚未得到充分研究。在此，我们表明，芜菁花叶病毒（TuMV）感染会下调拟南芥（Arabidopsis thaliana）中LHCB3的表达。当通过烟草脆裂病毒诱导的基因沉默使LHCB3沉默时，TuMV的系统感染受到抑制。在LHCB3沉默的植物中过氧化氢（H₂O₂）过度积累。抑制或消除活性氧（ROS）的化学处理削弱了LHCB3沉默植物对TuMV感染的抗性。将LHCB3与参与ROS产生的基因共同沉默会损害植物对TuMV的抗性，但将LHCB3与ROS清除途径中的基因共同沉默会增加对该病毒 的抗性。过表达LHCB3的转基因植物对TuMV更敏感。这些结果表明，LHCB3的下调通过诱导ROS产生参与对TuMV的防御。
