Post Graduation Program in Biosciences and Physiopathology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
Rheumatology Division, Department of Medicine, State University of Maringá, Paraná, Brazil.
Front Immunol. 2021 Jul 5;12:653611. doi: 10.3389/fimmu.2021.653611. eCollection 2021.
Ankylosing spondylitis (AS) is a chronic autoimmune inflammatory disease that mainly affects the axial and sacroiliac joints. Single-nucleotide polymorphisms (SNPs) in genes encoding cytokines have been associated with AS, which can interfere with the production of these cytokines and contribute to the development of AS. In order to contribute to a better understanding of the pathology of AS, our objective was to investigate a possible association of the -1082 A>G SNP (rs1800896) with AS and to evaluate the serum levels of TNF-α, IL-10, IL-17A, and IL-17F in AS patients and controls comparing them with their respective genotypes ( rs1800629, rs1800896, rs2275913, and rs763780). Patients and controls were selected from the Maringá University Hospital and the Maringá Rheumatism Clinic, in Paraná State, Southern Brazil, and they were diagnosed by the ASAS Criteria. In total, 149 patients and 169 controls were genotyped for the -1082 A>G polymorphism using a polymerase chain reaction with sequence specific primers (PCR-SSP); the measurement of TNF-α serum levels was performed through the immunofluorimetric test and IL-10, IL-17A, and IL-17F using an ELISA test. There was a high frequency of the -1082 G allele in AS patients compared with controls with an odds ratio of 1.83 and 95% confidence interval of 1.32 to 2.54, and a significant difference in the genotype frequencies of the -1082 A/G+G/G between patients and healthy controls, with an odds ratio of 3.01 and 95% confidence interval of 1.75 to 5.17. In addition, increased serum levels of IL-10 were observed in AS patients: 2.38 (IQR, 0.91) pg/ml compared with controls 1.72 (IQR 0.93) pg/ml (P = 0.01). Our results also showed an association between rs763780 genotypes and increased serum levels of IL-17F in patients with AS and also in controls. We can conclude that patients with the A/G and G/G genotypes for -1082 A>G (rs1800896) in the gene are three times more likely to develop AS, that the serum level of IL-10 was higher in AS patients and that the rs763780 polymorphism can affect the levels of IL-17F in the serum of patients and controls in the same way.
强直性脊柱炎（AS）是一种主要影响轴性和骶髂关节的慢性自身免疫性炎症性疾病。编码细胞因子的基因中的单核苷酸多态性（SNP）与 AS 相关，这可能干扰这些细胞因子的产生，并有助于 AS 的发展。为了更好地了解 AS 的病理学，我们的目标是研究 -1082 A>G SNP（rs1800896）与 AS 之间的可能关联，并评估 AS 患者和对照组中 TNF-α、IL-10、IL-17A 和 IL-17F 的血清水平，并将其与各自的基因型（rs1800629、rs1800896、rs2275913 和 rs763780）进行比较。患者和对照组均来自巴西南部巴拉那州马兰加大学医院和马兰加风湿病诊所，通过 ASAS 标准进行诊断。总共对 149 名患者和 169 名对照组进行了 -1082 A>G 多态性的聚合酶链反应与序列特异性引物（PCR-SSP）基因分型；TNF-α 血清水平的测定采用免疫荧光法，IL-10、IL-17A 和 IL-17F 采用 ELISA 法进行测定。与对照组相比，AS 患者中 -1082 G 等位基因的频率较高，比值比为 1.83，95%置信区间为 1.32 至 2.54， -1082 A/G+G/G 基因型频率在患者和健康对照组之间存在显著差异，比值比为 3.01，95%置信区间为 1.75 至 5.17。此外，AS 患者的血清 IL-10 水平升高：2.38（IQR，0.91）pg/ml 与对照组 1.72（IQR，0.93）pg/ml（P=0.01）。我们的结果还表明，rs763780 基因型与 AS 患者和对照组中 IL-17F 血清水平升高之间存在关联。我们可以得出结论， -1082 A>G（rs1800896）基因的 A/G 和 G/G 基因型的患者患 AS 的可能性增加三倍，AS 患者的血清 IL-10 水平较高，rs763780 多态性可以以相同的方式影响患者和对照组的血清中 IL-17F 的水平。
