Deng Zhuying, Wu Huiyan, Jin Tianlin, Cai Tingting, Jiang Mengting, Wang Mi, Liang Dacheng
Hubei Collaborative Innovation Center for Grain Industry, School of Agriculture, Yangtze University, Jingzhou, China.
Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, China.
Front Plant Sci. 2021 Jul 5;12:664342. doi: 10.3389/fpls.2021.664342. eCollection 2021.
Scion-rootstock union formation is a critical step toward the functional assemblage of heterogeneous plants. Interfamilial scion-rootstock interaction often results in graft incompatibility during the assemblage process, and the underlying mechanisms are largely unknown. In this study, we reported that tracheary element (TE) remodeling, including TE segmentation and deformation, rather than formation from callus or adjacent tissues, took place at the early stage of grafting interface between and (/). Following cellular deposits, the short TEs from both partners were overlapping, dependent on the homogeneity of contacting TEs, with each other. Without overlapping, the TEs at the interface would grow laterally, and the TEs above and below the interface would undergo self-fusion to form insulating spiraling bundles. Finally, the overlapping TEs constituted a continuous network through alignment. Our results provide a definitive framework for the critical process of TE behavior in the / distant grafts, including (1) segmentation and/or deformation, (2) matching, overlapping, and cellular deposits, and (3) aligning or spiraling. These insights might guide us in the future into constructing more compatible distant grafts from the perspective of TE homogeneity.
接穗 - 砧木结合是异质植物功能组装的关键步骤。在组装过程中，科间接穗 - 砧木相互作用常常导致嫁接不亲和，其潜在机制很大程度上尚不清楚。在本研究中，我们报道了管状分子（TE）重塑，包括TE分割和变形，而非由愈伤组织或相邻组织形成，发生在 和 （/）嫁接界面的早期阶段。细胞沉积后，来自双方的短TE相互重叠，这取决于接触TE的同质性。若不重叠，界面处的TE会横向生长，界面上方和下方的TE会进行自我融合以形成绝缘螺旋束。最后，重叠的TE通过排列构成连续网络。我们的结果为 / 远缘嫁接中TE行为的关键过程提供了一个明确的框架，包括（1）分割和/或变形，（2）匹配、重叠和细胞沉积，以及（3）排列或螺旋化。这些见解可能在未来指导我们从TE同质性的角度构建更兼容的远缘嫁接体。
