Bioscience and Biotechnology Center, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Science. 2020 Aug 7;369(6504):698-702. doi: 10.1126/science.abc3710.
Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue is attached to another through cell-cell adhesion. However, graft compatibility limits combinations to closely related species, and the mechanism is poorly understood. We found that is capable of graft adhesion with a diverse range of angiosperms. Comparative transcriptomic analyses on graft combinations indicated that a subclade of β-1,4-glucanases secreted into the extracellular region facilitates cell wall reconstruction near the graft interface. Grafting was promoted by overexpression of the β-1,4-glucanase. Using stem as an interscion, we produced tomato fruits on rootstocks from other plant families. These findings demonstrate that the process of cell-cell adhesion is a potential target to enhance plant grafting techniques.
植物嫁接是为了水果和蔬菜的繁殖，通过细胞间的黏附将一块活组织附着在另一个组织上。然而，嫁接的兼容性限制了组合到亲缘关系较近的物种，其机制也知之甚少。我们发现能够与多种被子植物进行嫁接黏附。对嫁接组合的比较转录组分析表明，分泌到细胞外区域的β-1,4-葡聚糖酶的一个亚类有助于在嫁接界面附近进行细胞壁重建。β-1,4-葡聚糖酶的过表达促进了嫁接。使用作为接穗，我们在来自其他植物科的砧木上生产了番茄果实。这些发现表明，细胞间黏附的过程是增强植物嫁接技术的潜在目标。
