Shahryari Marzieh, Bibak Bahram, Khaksari Mohammad, Keshavarzi Zakieh, Salmani Neda, Shirazpour Sara, Alimahdi Fatemeh
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.
Avicenna J Phytomed. 2021 Jul-Aug;11(4):380-393. doi: 10.22038/AJP.2021.17426.
Based on anti-inflammatory effects of , the effect of aqueous extract of this plant on brain edema and changes in some pro-inflammatory cytokines was investigated after traumatic brain injury (TBI).
In this study, adult male Wistar rats were divided into 5 groups: Sham, TBI, vehicle (Veh), and low dose (LA) and high dose (HA) . The vehicle and aqueous extract of were injected intraperitoneally 30 min after induction of diffuse TBI by Marmarou's method. Brain edema (brain water content), and transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6) and IL-1β levels in serum and brain were measured 24 hr after TBI induction.
Increased brain edema by TBI was reduced by both LA and HA (p<0.01 and p<0.05, respectively). IL-6 increased in the brain of TBI group compared to sham, and which was inhibited by both doses compared to Veh (p<0.001). The differences in the IL-6 serum levels among Veh, LA and HA groups were not significant. Increases in serum and brain IL-1β levels were reduced only in the HA group (p<0.001). Although only in the brain, TNF-α level increased after trauma, but both LA and HA inhibited it in a dose-dependent manner (p<0.01 and p<0.05, respectively) . The amount of TGF-β in the brain was reduced by both doses of the extract (p<0.001).
These results indicated that has a neuroprotective effect induced by reducing brain edema. The probable mechanism particularly for HA is decreasing levels of pro-inflammatory cytokines such as TGF-β, TNF-α, IL-6 and IL-1β.
基于[植物名称]的抗炎作用，研究该植物水提取物对创伤性脑损伤（TBI）后脑水肿及某些促炎细胞因子变化的影响。
在本研究中，成年雄性Wistar大鼠分为5组：假手术组、TBI组、溶剂对照组（Veh）、低剂量（LA）组和高剂量（HA）组。采用Marmarou法诱导弥漫性TBI 30分钟后，腹腔注射溶剂和[植物名称]水提取物。TBI诱导后24小时测量脑水肿（脑含水量）以及血清和脑中转化生长因子β（TGF-β）、肿瘤坏死因子α（TNFα）、白细胞介素6（IL-6）和IL-1β水平。
LA和HA均减轻了TBI引起的脑水肿增加（分别为p<0.01和p<0.05）。与假手术组相比，TBI组脑中IL-6增加，与Veh组相比，两个剂量的[植物名称]均抑制了IL-6增加（p<0.001）。Veh、LA和HA组之间血清IL-6水平差异不显著。仅HA组降低了血清和脑中IL-1β水平的增加（p<0.001）。虽然仅在脑中，创伤后TNF-α水平升高，但LA和HA均以剂量依赖性方式抑制了它（分别为p<0.01和p<0.05）。提取物的两个剂量均降低了脑中TGF-β的量（p<0.001）。
这些结果表明，[植物名称]通过减轻脑水肿具有神经保护作用。特别是HA的可能机制是降低促炎细胞因子如TGF-β、TNF-α、IL-6和IL-1β的水平。
