Soltani Zahra, Khaksari Mohammad, Jafari Elham, Iranpour Maryam, Shahrokhi Nader
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Physiol Behav. 2015 Dec 1;152(Pt A):26-31. doi: 10.1016/j.physbeh.2015.08.037. Epub 2015 Sep 11.
The concerns about negative consequences of estrogen therapy have led to introduce other strategies to obtain estrogen's benefits in the brain. The present study tests the hypothesis that a major isoflavone of soy; genistein with estrogen-like activity can be neuroprotective in traumatic brain injury (TBI). The male Wistar rats were randomly divided to four groups: sham, TBI, vehicle and genistein. The TBI was induced by Marmarou method. The brain edema and the disruption of blood-brain-barrier (BBB) were evaluated 48 h post-TBI. Genistein (15 mg/kg) or dimethyl sulfoxide (DMSO) was injected i.p., twice after TBI. The intracranial pressure (ICP), the motor performance, and the beam-walk task (WB) were determined before trauma, on trauma day (D0), and first (D1) and second (D2) days post-TBI. Genistein inhibited a development of brain edema and a BBB permeability in TBI animals. An increase of ICP and a defect in motor and WB performance were showed following TBI, in all times evaluated. An increase of ICP induced by TBI was suppressed by genistein on D1 and D2 times. Genistein improved a motor disorder induced by TBI, on D1 and D2 times. Also an increase of traversal time in WB task was suppressed by genistein in TBI animals, on D1 and D2 times. The results of this study demonstrated that genistein can be neuroprotective in TBI. Genistein inhibited the disruption of BBB, the brain edema and the increase of ICP, and the disturbance of neurobehavioral performance in TBI.
对雌激素疗法负面后果的担忧促使人们引入其他策略来获取雌激素对大脑的益处。本研究检验了这样一个假设：大豆的一种主要异黄酮——具有雌激素样活性的染料木黄酮，对创伤性脑损伤（TBI）具有神经保护作用。雄性Wistar大鼠被随机分为四组：假手术组、TBI组、溶剂对照组和染料木黄酮组。采用 Marmarou 法诱导TBI。在TBI后48小时评估脑水肿和血脑屏障（BBB）的破坏情况。TBI后经腹腔注射染料木黄酮（15 mg/kg）或二甲基亚砜（DMSO），注射两次。在创伤前、创伤当天（D0）以及TBI后的第一天（D1）和第二天（D2）测定颅内压（ICP）、运动能力和横梁行走任务（WB）。染料木黄酮抑制了TBI动物脑水肿的发展和BBB通透性。在所有评估时间点，TBI后均出现ICP升高以及运动和WB表现缺陷。TBI诱导的ICP升高在D1和D2时间点被染料木黄酮抑制。染料木黄酮在D1和D2时间点改善了TBI诱导的运动障碍。此外，在D1和D2时间点，染料木黄酮抑制了TBI动物在WB任务中穿越时间的增加。本研究结果表明，染料木黄酮对TBI具有神经保护作用。染料木黄酮抑制了TBI中BBB的破坏、脑水肿、ICP升高以及神经行为表现的紊乱。
