芦荟和硫糖铝对大鼠胃微循环变化、细胞因子水平及胃溃疡愈合的影响。
Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats.
作者信息
Eamlamnam Kallaya, Patumraj Suthiluk, Visedopas Naruemon, Thong-Ngam Duangporn
机构信息
Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
出版信息
World J Gastroenterol. 2006 Apr 7;12(13):2034-9. doi: 10.3748/wjg.v12.i13.2034.
AIM
To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing.
METHODS
Male Spraque-Dawley rats (n=48) were divided into four groups. Group1 served as control group, group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-alpha and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid.
RESULTS
On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P<0.05) increased in the ulcer groups when compared to the control group. The level of TNF-alpha was elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera, leukocyte adherence was reduced in postcapillary venule. The level of IL-10 was elevated, but the level of TNF-alpha had no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-alpha were still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-alpha level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment.
CONCLUSION
Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-alpha level and reduce IL-10 level. Aloe vera treatment can reduce leukocyte adherence and TNF-alpha level, elevate IL-10 level and promote gastric ulcer healing.
目的
比较芦荟和硫糖铝对胃微循环变化、细胞因子水平及胃溃疡愈合的影响。
方法
将48只雄性Spraque-Dawley大鼠分为四组。第1组作为对照组，第2组为未治疗的胃溃疡组，第3组和第4组为分别用硫糖铝和芦荟治疗的胃溃疡组。每组大鼠再分为两个亚组，分别于用20%乙酸诱导胃溃疡后的第1天和第8天研究白细胞黏附、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）水平以及胃溃疡愈合情况。
结果
在诱导胃溃疡后的第1天，与对照组相比，溃疡组毛细血管后微静脉中的白细胞黏附显著增加（P<0.05）。TNF-α水平升高，IL-10水平降低。在用硫糖铝和芦荟治疗的溃疡组中，毛细血管后微静脉中的白细胞黏附减少。IL-10水平升高，但TNF-α水平无显著差异。在第8天，未治疗的溃疡组中毛细血管后微静脉中的白细胞黏附和TNF-α水平仍然升高，IL-10水平降低。用硫糖铝和芦荟治疗的溃疡组毛细血管后微静脉中的白细胞黏附和TNF-α水平较低。与未治疗的溃疡组相比，IL-10水平仍然升高。此外，在诱导胃溃疡后的第1天和第8天对胃进行组织病理学检查显示，胃组织受损并伴有炎症。在第1天和第8天用硫糖铝和芦荟治疗的溃疡组中，胃炎症减轻，上皮细胞增殖增强，胃腺变长。与未治疗的溃疡组相比，溃疡大小也减小。
结论
给予20%乙酸可诱发胃炎症，增加毛细血管后微静脉中的白细胞黏附和TNF-α水平，并降低IL-10水平。芦荟治疗可减少白细胞黏附和TNF-α水平，升高IL-10水平并促进胃溃疡愈合。
Zotero 插件