Gao Qi, Zheng Zezhong, Wang Heng, Yi Songqiang, Zhang Guihong, Gong Lang
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, China.
Front Vet Sci. 2021 Jul 5;8:697839. doi: 10.3389/fvets.2021.697839. eCollection 2021.
On October 30, 2020, piglets and sows in the farrowing house of a pig farm in Jiangxi showed clinical symptoms such as anorexia, watery diarrhea, and vomiting. Epidemiological test, clinical necropsy, and RT-PCR test were carried out on the pig farm for diagnosis. After comprehensive considerations, the disease was judged as porcine epidemic diarrhea virus infection. Thereafter, a series of comprehensive prevention and control measures such as emergency vaccination with autogenous vaccines were adopted. Half a month after inoculation with autogenous vaccines for the farm, the mortality rate of newborn piglets in the farrowing house began to decline, and production gradually returned to being stable. The second-generation sequencing analysis and phylogenetic analysis showed that the porcine epidemic diarrhea virus (PEDV) sequence obtained from the stool and small intestine samples of the diseased pigs on the farm was 97.8% homologous to the vaccine strain. At the same time, antibody testing found that the vaccinated pigs on the pig farm had satisfactory immune response. This case indicated that the PEDV outbreak on the pig farm might aggravate owing to the strain being mutated and could escape the immune protection of the existing vaccine. This case has accumulated technical data for the clinical prevention and control of porcine epidemic diarrhea.
2020年10月30日，江西某猪场产房的仔猪和母猪出现厌食、水样腹泻、呕吐等临床症状。对该猪场进行了流行病学调查、临床剖检及RT-PCR检测以进行诊断。综合考虑后，判定该病为猪流行性腹泻病毒感染。此后，采取了一系列综合防控措施，如用自制疫苗进行紧急接种。该猪场接种自制疫苗半个月后，产房新生仔猪死亡率开始下降，生产逐渐恢复稳定。二代测序分析及系统发育分析表明，从该猪场患病猪粪便和小肠样本中获得的猪流行性腹泻病毒（PEDV）序列与疫苗株的同源性为97.8%。同时，抗体检测发现该猪场接种疫苗的猪免疫反应良好。该病例表明，猪场PEDV疫情可能因毒株变异而加重，并可能逃避现有疫苗的免疫保护。该病例为猪流行性腹泻的临床防控积累了技术资料。
