State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, Sichuan, China.
Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China.
Chem Commun (Camb). 2021 Aug 12;57(65):8039-8042. doi: 10.1039/d1cc03011f.
Two-dimensional (2D) hexagonal boron nitride nanosheet (h-BNNS) is proposed as an effective nanoquencher for fluorescence detection of biocompatible microRNA. Compared with bulk hexagonal boron nitride (h-BN), the exfoliated ultrathin nanosheet has a narrow band gap and increased conductivity, thus enabling fast electron transfer with this electron acceptor for more effective fluorescence detection of microRNA. Remarkably, using the nanoprobe consisting of h-BNNS and FAM dye-labeled ssDNA, a low detection limit of 2.39 nM is achieved and a rapid fluorescence response is observed compared with previously reported fluorescence sensing materials. More importantly, this sensing system could also distinguish base-mismatched microRNA, suggesting that the proposed sensing platform held excellent selectivity and great promise for application in the detection of nucleotide polymorphism. This work will benefit microRNA-related fundamental research and disease diagnosis.
二维（2D）六方氮化硼纳米片（h-BNNS）被提议作为一种有效的纳米猝灭剂，用于生物相容性 microRNA 的荧光检测。与体相六方氮化硼（h-BN）相比，剥离的超薄纳米片具有更窄的带隙和更高的导电性，从而能够与这种电子受体进行快速的电子转移，从而更有效地检测 microRNA 的荧光。值得注意的是，使用由 h-BNNS 和 FAM 染料标记的 ssDNA 组成的纳米探针，与之前报道的荧光传感材料相比，实现了低至 2.39 nM 的检测限和快速的荧光响应。更重要的是，该传感系统还可以区分碱基错配的 microRNA，表明所提出的传感平台具有优异的选择性，有望应用于核苷酸多态性的检测。这项工作将有益于 microRNA 相关的基础研究和疾病诊断。
