Department of Medical Oncology, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China.
Nursing Department, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China.
Histol Histopathol. 2022 Feb;37(2):117-124. doi: 10.14670/HH-18-360. Epub 2021 Jul 22.
Lot of attention had been paid to the role of circular RNAs (circRNAs) in carcinogenesis recently. However, knowledge about circRNAs in NSCLC development is far from satisfactory. In this study, we aimed to provide a novel insight into the circRIP2 in NSCLC development. We used NSCLC tissues, as well as cell lines to elucidate the expression and location of circRIP2 in NSCLC. We also established the circRIP2 overexpression cells A549-circRIP2 and repression cells HCC827-shcircRIP2 for further functional and mechanism studies. The pro-tumorigenic role of circRIP2 was tested by using CCK-8, BrdU and transwell assays. The interaction between circRIP2 and miR-671-5p were validated by luciferase reporter assay, RIP assay, as well as RNA pull down assay. We showed circRIP2 is differentially expressed NSCLC, and acted as a predictor for overall survival (OS) and disease-free survival (DFS). CircRIP2 promoted NSCLC progression by acting as a miRNA sponge for miR-671-5p, thus facilitating its target gene FOXM1 expression. Targeting circRIP2 could be potentially beneficial for NSCLC patients in the future.
最近，人们对环状 RNA（circRNAs）在癌症发生中的作用给予了大量关注。然而，关于非小细胞肺癌（NSCLC）发展中 circRNAs 的知识还远远不能令人满意。在这项研究中，我们旨在为 circRIP2 在 NSCLC 发展中的作用提供新的见解。我们使用 NSCLC 组织和细胞系来阐明 circRIP2 在 NSCLC 中的表达和定位。我们还建立了 circRIP2 过表达细胞系 A549-circRIP2 和抑制表达细胞系 HCC827-shcircRIP2，以进行进一步的功能和机制研究。通过 CCK-8、BrdU 和 Transwell 实验测试了 circRIP2 的促肿瘤作用。通过荧光素酶报告实验、RIP 实验和 RNA 下拉实验验证了 circRIP2 与 miR-671-5p 之间的相互作用。我们显示 circRIP2 在 NSCLC 中差异表达，并作为总生存期（OS）和无病生存期（DFS）的预测因子。circRIP2 通过作为 miR-671-5p 的 miRNA 海绵，促进 NSCLC 进展，从而促进其靶基因 FOXM1 的表达。未来针对 circRIP2 可能对 NSCLC 患者有益。
