Kulikouskaya Viktoryia, Zhdanko Tsimafei, Hileuskaya Kseniya, Kraskouski Aliaksandr, Zhura Alexandr, Skorohod Hennadiy, Butkevich Vasili, Pal Kunal, Tratsyak Stanislau, Agabekov Vladimir
Laboratory of micro- and nanostructured systems, Institute of chemistry of new materials National academy of sciences of Belarus, 36 F. Skaryna str, Minsk, 220141, Belarus.
Department of Surgical Diseases, Belorussian State Medical University, 83 Dzerzhinski Ave, Minsk, 220116, Belarus.
J Biomed Mater Res A. 2022 Jan;110(1):217-228. doi: 10.1002/jbm.a.37278. Epub 2021 Jul 22.
Implant-related infection is one of the serious problems in regenerative medicine. Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly. In this study, multilayer films based on biopolymers (pectin and chitosan) and their nanocomposites with silver nanoparticles have been prepared and evaluated. The buildup of multilayers was monitored using the quartz crystal microbalance with dissipation technique. The morphology of the obtained films was investigated by atomic force microscopy. We have demonstrated that pectin-Ag-containing films were characterized by the linear growth and smooth defect-free surface. When pectin-Ag was substituted for the pectin in the multilayer systems, the properties of the formed coatings were significantly changed: the film rigidity and surface roughness increased, as well as the film growth acquired the parabolic character. All prepared multilayer films have shown antibacterial activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. The significant decrease in the number of the adhered E. coli on the multilayer surface has been determined; moreover, many of the cells were misshapen with cytoplasm leaking. The prepared multilayer films showed a mild activity against S. aureus predominantly due to the antiadhesive effect. Our results indicate that antibacterial activity of biopolymer multilayers is determined by the film composition and physicochemical characteristics and can be associated with their antiadhesive and bactericidal behaviors.
植入物相关感染是再生医学中的严重问题之一。克服医用植入物上细菌生长所引起问题的一种有前景的方法是通过生物活性涂层对其进行改性。逐层组装是一种在纳米尺度上设计具有定制特性的多层膜的通用技术。在本研究中，基于生物聚合物（果胶和壳聚糖）及其与银纳米颗粒的纳米复合材料的多层膜已被制备并评估。使用具有耗散技术的石英晶体微天平监测多层膜的形成过程。通过原子力显微镜研究所得薄膜的形态。我们已经证明，含果胶 - 银的薄膜具有线性生长和光滑无缺陷表面的特征。当在多层体系中用果胶 - 银替代果胶时，形成的涂层性能发生了显著变化：薄膜刚性和表面粗糙度增加，并且薄膜生长呈现抛物线特征。所有制备的多层膜均对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌）表现出抗菌活性。已确定多层膜表面上粘附的大肠杆菌数量显著减少；此外，许多细胞畸形且细胞质泄漏。所制备的多层膜对金黄色葡萄球菌表现出温和的活性，主要归因于抗粘附作用。我们的结果表明，生物聚合物多层膜的抗菌活性由薄膜组成和物理化学特性决定，并且可能与其抗粘附和杀菌行为相关。
