G.N. Gabrichevsky Research Institute of Epidemiology and Microbiology.
State Research Center for Applied Microbiology and Biotechnology.
Klin Lab Diagn. 2021 Jul 16;66(7):428-437. doi: 10.51620/0869-2084-2021-66-7-428-437.
The purpose of the work is to evaluate the cultural and morphological properties of colonies of clinically significant corynebacteria on culture mediums for the isolation of corynebacteria. The study used 9 culture mediums for the isolation of corynebacteria: a culture medium for the isolation of corynebacteria (Corynebacagar); Tellurite-containing blood agars on base - Culture medium № 1 GRM, Culture agar for the cultivation of microorganisms (GRM agar), Culture medium for determining the sensitivity of microorganisms to antibacterial preparations - AGV, culture agar for the cultivation of dry microorganisms (SPA), Clauberg medium II, Hoyle Medium agar (Oxoid), Blood agar base (Conda), Columbia Agar Base (Conda). The work used 7 test strains of microorganisms from the State collections of pathogenic microorganisms - C. diphtheriae biovars gravis, mitis, intermedius, belfanti and subspecies lausannense, C. ulcerans and C.pseudotuberculosis. Studies were carried out in accordance with MUK 4.2.3065-13 «Laboratory diagnosis of diphtheria infection». We describe culture-morphological properties of strains on all tested culture mediums the isolation of corynebacteria after 24 and 48 hours of incubation. Analysis of the results on the growth properties of culture mediums showed that all culture mediums had high sensitivity - from dilution 10-7 for all test strains. Colonies of corynebacteria were visually detected on culture mediums after 19-20 hours of cultivation. When cultivating a suspension of corynebacteria from breeding 10-6 on culture mediums, the number of colonies ranged from 95±5 to 120±10. Conclusion. All culture mediums had differential diagnostic properties that ensure the growth of corynebacteria after the day of incubation.
本工作旨在评估临床上有意义的棒状杆菌属在棒状杆菌属分离培养基上的文化和形态特性。该研究使用了 9 种用于分离棒状杆菌的培养基：棒状杆菌分离培养基（Corynebacagar）；基于基础的含碲血琼脂 - 培养基№ 1 GRM、微生物培养琼脂（GRM 琼脂）、用于测定微生物对抗菌制剂敏感性的培养基 - AGV、干微生物培养琼脂（SPA）、Clauberg 培养基 II、Hoyle 培养基琼脂（Oxoid）、血琼脂基础（Conda）、哥伦比亚琼脂基础（Conda）。该工作使用了 7 株来自国家致病性微生物收藏库的测试菌株 - C. 白喉棒状杆菌生物型重、中、轻、belfanti 和lausannense亚种、C. 溃疡棒状杆菌和 C.pseudotuberculosis。研究是按照 MUK 4.2.3065-13“白喉感染的实验室诊断”进行的。我们描述了在所有测试的分离棒状杆菌的培养基上菌株的培养形态特性，在孵育 24 和 48 小时后。对培养基生长特性的结果分析表明，所有培养基的敏感性都很高 - 从所有测试菌株的 10-7 稀释度。在培养 19-20 小时后，在培养基上肉眼可以检测到棒状杆菌的菌落。当在培养基上从 10-6 的繁殖物培养棒状杆菌悬浮液时，菌落数从 95±5 到 120±10 不等。结论。所有培养基均具有鉴别诊断特性，可确保在孵育日后棒状杆菌的生长。
