G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology.
State Research Center for Applied Microbiology and Biotechnology.
Klin Lab Diagn. 2021 Aug 13;66(8):509-512. doi: 10.51620/0869-2084-2021-66-8-509-512.
The results of comparative experimental studies of identification of nontoxigenic C. diphtheriae strain by three different commercial laboratories are presented. A typical nontoxigenic strain of C. diphtheriae biovar mitis was used. For the studies, three lines of ten-fold dilutions of bacterial culture were prepared, followed by control planting on the medium and counting CFU/ml. In the experiment, tampons were pooled with a 24-hour bacterial culture of a nontoxigenic C. diphtheriae strain. Tampons were provided from three different laboratories - ∑-Transwab® with Ames liquid medium (from the first and second laboratories) and a viscose tampon with coal medium (from the third laboratory). After pooled, tampons were delivered to commercial laboratories. And as a result of the experiment, Corynebacterium spp. was identified in first laboratory (103 CFU/tamp), S. epidermidis (102 CFU/ml) - in second laboratory and nontoxigenic C. diphtheriae biovar gravis - in third laboratory. The study indicates that there is a need to the supervision of bacteriological investigations conducted in various laboratories. This will improve the quality of investigations on diphtheria infection and identify of diphtheria carrier, which is a reservoir of the causative agent of diphtheria, and will contribute to the maintenance of sanitary and epidemiological well-being in our country.
介绍了三家不同商业实验室对非产毒白喉棒状杆菌鉴定的对比实验研究结果。使用了典型的非产毒白喉棒状杆菌生物型温和株。在研究中，制备了细菌培养物的十倍系列稀释液三条线，随后在培养基上进行对照种植并计算 CFU/ml。在实验中，将带有 24 小时非产毒白喉棒状杆菌细菌培养物的棉拭子混合在一起。棉拭子来自三个不同的实验室——∑-Transwab®含 Ames 液体培养基（来自第一和第二实验室）和含煤的粘胶棉拭子（来自第三实验室）。混合后，将棉拭子送到商业实验室。实验结果表明，第一实验室鉴定出棒状杆菌属 spp.（103 CFU/拭子），第二实验室鉴定出表皮葡萄球菌（102 CFU/ml），第三实验室鉴定出非产毒白喉棒状杆菌生物型重型。该研究表明，有必要对各个实验室进行的细菌学调查进行监督。这将提高白喉感染调查和白喉带菌者鉴定的质量，白喉带菌者是白喉病原体的储存库，有助于维护我国的卫生和流行病学健康。
