铵作为氮源对顶头孢霉C-10生产δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸合成酶的影响。
Effect of ammonium as nitrogen source on production of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase by Cephalosporium acremonium C-10.
作者信息
Zhang J Y, Wolfe S, Demain A L
机构信息
Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
J Antibiot (Tokyo). 1987 Dec;40(12):1746-50. doi: 10.7164/antibiotics.40.1746.
Cephalosporin production by Cephalosporium acremonium strain C-10 was suppressed when the organic nitrogen source (1.2% L-asparagine) was replaced by 1.2% (NH4)2SO4. A higher level of (NH4)2SO4 (3.5%) led to even greater suppression. Ammonium repression was exerted on formation of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase, together with that of expandase; a lesser effect by ammonium was observed on cyclase production. Inhibition of ACV synthetase activity byammonium was also observed (ca. 50% inhibition at 250 mM NH4+).
当用1.2%的硫酸铵替换有机氮源（1.2%的L-天冬酰胺）时，顶头孢霉C-10菌株的头孢菌素产量受到抑制。更高水平的硫酸铵（3.5%）导致更大程度的抑制。铵阻遏作用于δ-（L-α-氨基己二酰基）-L-半胱氨酰-D-缬氨酸（ACV）合成酶以及扩展酶的形成；铵对环化酶产生的影响较小。还观察到铵对ACV合成酶活性的抑制作用（在250 mM NH4+时约50%的抑制率）。
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