Impact of anesthesia on static and dynamic functional connectivity in mice.

A few studies have compared the static functional connectivity between awake and lightly anesthetized states in rodents by resting-state fMRI. However, impact of light anesthesia on static and dynamic fluctuations in functional connectivity has not been fully understood. Here, we developed a resting-state fMRI protocol to perform awake and anesthetized functional MRI in the same mice. Static functional connectivity showed a widespread decrease under light anesthesia, such as when under isoflurane or a mixture of isoflurane and medetomidine. Several interhemispheric and subcortical connections were key connections for anesthetized condition from awake state. Dynamic functional connectivity demonstrates the shift from frequent broad connections across the cortex, the hypothalamus, and the auditory-visual cortex to frequent local connections within the cortex only under light anesthesia compared with awake state. Fractional amplitude of low frequency fluctuation in the thalamic nuclei decreased under both anesthesia. These results indicate that typical anesthetics for functional MRI alters the spatiotemporal profile of the dynamic brain network in subcortical regions, including the thalamic nuclei and limbic system.