Research & Development Center of Blueberry, Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, 35 QinghuaEast Road, 100083, Beijing, China.
BMC Genomics. 2021 Jul 22;22(1):565. doi: 10.1186/s12864-021-07850-5.
Blueberry (Vaccinium corymbosum L.) is an important species with a high content of flavonoids in fruits. As a perennial shrub, blueberry is characterized by shallow-rooted property and susceptible to drought stress. MYB transcription factor was reported to be widely involved in plant response to abiotic stresses, however, the role of MYB family in blueberry responding to drought stress remains elusive.
In this study, we conducted a comprehensive analysis of VcMYBs in blueberry based on the genome data under drought stress, including phylogenetic relationship, identification of differentially expressed genes (DEGs), expression profiling, conserved motifs, expression correlation and protein-protein interaction prediction, etc. The results showed that 229 non-redundant MYB sequences were identified in the blueberry genome, and divided into 23 subgroups. A total of 102 MYB DEGs with a significant response to drought stress were identified, of which 72 in leaves and 69 in roots, and 8 differential expression genes with a > 20-fold change in the level of expression. 17 DEGs had a higher expression correlation with other MYB members. The interaction partners of the key VcMYB proteins were predicted by STRING analysis and in combination with physiological and morphological observation. 10 key VcMYB genes such as VcMYB8, VcMYB102 and VcMYB228 were predicted to be probably involved in reactive oxygen species (ROS) pathway, and 7 key VcMYB genes (VcMYB41, VcMYB88 and VcMYB100, etc..) probably participated in leaf regulation under drought treatment.
Our studies provide a new understanding of the regulation mechanism of VcMYB family in blueberry response to drought stress, and lay fundamental support for future studies on blueberry grown in regions with limited water supply for this crop.
蓝莓（Vaccinium corymbosum L.）是一种重要的水果，其果实中含有丰富的类黄酮。作为一种多年生灌木，蓝莓具有浅根性和易受干旱胁迫的特点。MYB 转录因子广泛参与植物对非生物胁迫的响应，然而，MYB 家族在蓝莓响应干旱胁迫中的作用仍不清楚。
本研究基于蓝莓基因组数据，对干旱胁迫下的 VcMYBs 进行了综合分析，包括系统发育关系、差异表达基因（DEGs）鉴定、表达谱分析、保守基序、表达相关性和蛋白质-蛋白质相互作用预测等。结果表明，在蓝莓基因组中鉴定出 229 个非冗余 MYB 序列，分为 23 个亚组。共鉴定到 102 个 MYB DEGs 对干旱胁迫有显著响应，其中叶片 72 个，根 69 个，表达水平变化超过 20 倍的差异表达基因 8 个。17 个 DEG 与其他 MYB 成员的表达相关性较高。通过 STRING 分析和结合生理形态观察预测关键 VcMYB 蛋白的相互作用伙伴。预测 10 个关键 VcMYB 基因（如 VcMYB8、VcMYB102 和 VcMYB228）可能参与活性氧（ROS）途径，7 个关键 VcMYB 基因（如 VcMYB41、VcMYB88 和 VcMYB100 等）可能参与干旱处理下的叶片调节。
本研究为蓝莓响应干旱胁迫中 VcMYB 家族的调控机制提供了新的认识，为未来在水资源有限的地区种植蓝莓的研究奠定了基础。
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