Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2019 Feb 15;20(4):855. doi: 10.3390/ijms20040855.
Chinese chestnut ( Blume) can be infested by Yasumatsu, resulting in gall formation and yield losses. Research on the control of gall wasps using genomics approaches is rarely reported. We used RNA-seq to investigate the dynamic changes in the genes of a chestnut species ( B.) during four gall-formation stages caused by . A total of 21,306 genes were annotated by BLAST in databases. Transcriptome comparison between different gall-formation stages revealed many genes that were differentially expressed compared to the control. Among these, 2410, 7373, 6294, and 9412 genes were differentially expressed in four gall-formation stages: initiation stage (A), early growth stage (B), late growth stage (C), and maturation stage (D), respectively. Annotation analysis indicated that many metabolic processes (e.g., phenylpropanoid biosynthesis, secondary metabolism, plant⁻pathogen interaction) were affected. Interesting genes encoding putative components of signal transduction, stress response, and transcription factors were also differentially regulated. These genes might play important roles in response to gall formation. These new data on the mechanism by which infests chestnuts could help improve chestnut resistance.
栗瘿蜂可以为害板栗，导致瘿瘤形成和产量损失。利用基因组学方法研究瘿蜂的防治方法鲜有报道。我们利用 RNA-seq 技术研究了栗属植物（B.）在被栗瘿蜂为害的四个瘿瘤形成阶段的基因动态变化。通过 BLAST 在数据库中注释了总共 21306 个基因。不同瘿瘤形成阶段的转录组比较显示，与对照相比，许多基因差异表达。其中，在四个瘿瘤形成阶段（A、B、C 和 D）中，分别有 2410、7373、6294 和 9412 个基因差异表达。注释分析表明，许多代谢过程（如苯丙烷生物合成、次生代谢、植物-病原体相互作用）受到影响。有趣的是，编码信号转导、应激反应和转录因子的假定成分的基因也被差异调控。这些基因可能在响应栗瘿蜂的形成中发挥重要作用。这些关于栗瘿蜂为害板栗机制的新数据可能有助于提高板栗的抗性。
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