Fabrication of a multi-level drug release platform with liposomes, chitooligosaccharides, phospholipids and injectable chitosan hydrogel to enhance anti-tumor effectiveness.

Some anti-cancer drugs have poor solubility and availability, and are easily eliminated by rapid metabolism in vivo. To fix the drugs at the administration site and delay their release, a release platform with multi-level and multi-function was designed. The results showed that the curcumin (Cur) loaded liposomes (Cur@Lip) were coated sequentially with positive Chitooligosaccharides (Cur@Lip-Cos) and negative phospholipids (Cur@Lip-Cos-PC), to enhance water solubility, encapsulation efficiency, and delayed the release of the Cur, stability and cell intake of the liposomes, and the bioactivity of the system. The Cur@Lip-Cos could significantly enhance the inhibitory effect of MCF-7, better than the Cur@Lip-Cos-PC. The Lips were then fixed in an injectable thiolated chitosan hydrogel for local immobilization and sustained release which can effectively delay the release of Cur to inhibit MCF-7 growth. In summary, the innovative and biomimetic liposomal hydrogels are expected to provide more ideas for the design of drug carriers.