De Miguel I, Puech-Costes E, Samain D
Centre de Transfert en Biotechnologie-Microbiologie (INSA), Toulouse, France.
J Chromatogr. 1987 Oct 16;407:109-19. doi: 10.1016/s0021-9673(01)92608-1.
The possibility of using mixed ion-pair reversed-phase high-performance liquid chromatography for the preparative purification of aminoglycoside antibiotics was investigated. Retention can be satisfactorily defined in aqueous conditions but efficiency is critical. The analysis of a polynominal model allowed ion-pairing agents compositions to be designed leading to improved efficiency. Inexpensive aqueous mobile phases using volatile buffers can be defined for the satisfactory ion-pair preparative chromatography of aminoglycosides.
研究了使用混合离子对反相高效液相色谱法对氨基糖苷类抗生素进行制备纯化的可能性。在水性条件下可以令人满意地确定保留情况，但效率至关重要。通过对多项式模型的分析，可以设计离子对试剂的组成以提高效率。对于氨基糖苷类的令人满意的离子对制备色谱，可以定义使用挥发性缓冲剂的廉价水性流动相。
