Krainer E, Khare R K, Naider F, Becker J M
Anal Biochem. 1987 Jan;160(1):233-9. doi: 10.1016/0003-2697(87)90635-x.
A novel mode of reversed-phase high-performance liquid chromatography in which the mobile phase reacts chemically with the compounds to be separated was developed. Nikkomycin X and nikkomycin Z, two natural isomeric nucleoside peptide antibiotics, move as a single peak on a C18 reversed-phase column using an aqueous trifluoroacetic acid mobile phase. Addition of sodium bisulfite (1.0%) to the mobile phase results in the formation of a polar bisulfite addition product with nikkomycin X, but not with nikkomycin Z, inside the HPLC column. This type of reactive chromatography, or chemochromatography, led to the analytical and preparative separation of nikkomycins X and Z which are normally very intractable to separation by conventional chromatographic techniques.
开发了一种新型反相高效液相色谱模式，其中流动相与待分离化合物发生化学反应。尼可霉素X和尼可霉素Z是两种天然的异构核苷肽抗生素，在使用三氟乙酸水溶液流动相的C18反相柱上以单峰形式移动。向流动相中加入亚硫酸氢钠（1.0%）会导致在HPLC柱内与尼可霉素X形成极性亚硫酸氢盐加成产物，但与尼可霉素Z不反应。这种类型的反应色谱法，即化学色谱法，实现了尼可霉素X和Z的分析和制备分离，而这两种物质通常很难用传统色谱技术分离。
