George Philip, Mani Suresh, Abraham Priya, Michael Rajiv C
Department of Head and Neck surgery, Christian Medical College Hospital, Vellore, 632004 India.
Department of Clinical Virology, Christian Medical College Hospital, Vellore, 632004 India.
Indian J Surg Oncol. 2021 Jun;12(2):306-310. doi: 10.1007/s13193-020-01127-1. Epub 2020 Jun 13.
Various etiological factors have been described in laryngeal carcinogenesis. Tobacco and alcohol play a major role in the development of laryngeal cancers. However, recently there has been an overshadowing of association of human papillomavirus infection in laryngeal cancers. The aim of this study is to assess the prevalence of human papillomavirus (HPV) in malignant laryngeal lesions. This is a case control study. We conducted this study using tumor tissue specimens from 30 laryngeal squamous cell carcinoma patients and benign laryngeal tissue specimens from 30 cancer-free controls. The specimens from the lesion were sent to histopathological analysis as well as DNA extraction (DNeasy® Tissue kit) and polymerase chain reaction for detection of HPV. Positive samples underwent sequencing to detect the HPV serotype. Statistical analysis was performed using SPSS software. All 30 benign laryngeal lesion specimens were negative for the HPV DNA, while 4 of the 30 (7.5%) squamous cell carcinoma lesions were positive. One was HPV 16 and another one HPV 11. Two positive cases were not able to be sequenced probably due to low viral load. Our study suggests that the proportion of laryngeal squamous cell carcinomas attributable to infection by HPV seems to be low. Further prospective studies should be conducted with a larger group of patients to confirm the role of HPV in laryngeal cancers especially in treatment response and survival.
在喉癌发生过程中，已描述了多种病因。烟草和酒精在喉癌的发展中起主要作用。然而，最近人乳头瘤病毒感染与喉癌的关联已变得更为突出。本研究的目的是评估人乳头瘤病毒（HPV）在恶性喉病变中的患病率。这是一项病例对照研究。我们使用来自30例喉鳞状细胞癌患者的肿瘤组织标本和来自30例无癌对照的良性喉组织标本进行了这项研究。将病变标本送去进行组织病理学分析以及DNA提取（DNeasy®组织试剂盒）和聚合酶链反应以检测HPV。阳性样本进行测序以检测HPV血清型。使用SPSS软件进行统计分析。所有30例良性喉病变标本的HPV DNA均为阴性，而30例（7.5%）鳞状细胞癌病变中有4例为阳性。一例为HPV 16，另一例为HPV 11。两例阳性病例可能由于病毒载量低而无法测序。我们的研究表明，由HPV感染引起的喉鳞状细胞癌比例似乎较低。应使用更大规模的患者群体进行进一步的前瞻性研究，以证实HPV在喉癌中的作用，特别是在治疗反应和生存方面。
