用于鉴定粗球孢子菌分离株的Leathers-Awasthi培养基评估
Evaluation of Leathers-Awasthi medium for identifying Coccidioides immitis isolates.
作者信息
Standard P, Kaufman L
机构信息
Division of Mycotic Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1987 Dec;25(12):2428-9. doi: 10.1128/jcm.25.12.2428-2429.1987.
Leathers-Awasthi medium (LAM) has been recommended for the definitive identification of Coccidioides immitis isolates recovered from clinical specimens. Studies were undertaken to determine the diagnostic reliability of this medium for identifying fungal cultures. A total of 62 fungi, representing 13 C. immitis isolates and 49 similar pathogenic and saprophytic fungi, were tested in parallel by the LAM and exoantigen tests. All of the 13 C. immitis isolates were positive by the exoantigen test, whereas only 11 were positive on LAM. Of the 49 other fungal isolates, 29 were positive on LAM whereas none was positive by the exoantigen test. LAM does not permit the unequivocal identification of C. immitis isolates.
已推荐使用皮革-阿瓦斯蒂培养基（LAM）对从临床标本中分离出的粗球孢子菌进行最终鉴定。开展了多项研究以确定该培养基用于鉴定真菌培养物的诊断可靠性。通过LAM和外抗原试验对总共62种真菌进行了平行检测，其中包括13株粗球孢子菌分离株以及49种相似的致病真菌和腐生真菌。13株粗球孢子菌分离株在外抗原试验中均呈阳性，而在LAM上只有11株呈阳性。在49株其他真菌分离株中，29株在LAM上呈阳性，而在外抗原试验中无一呈阳性。LAM无法明确鉴定粗球孢子菌分离株。
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