Sekhon A S, DiSalvo A F, Standard P G, Kaufman L, Terreni A A, Garg A K
Am J Clin Pathol. 1984 Aug;82(2):206-9. doi: 10.1093/ajcp/82.2.206.
Commercially available individual serologic reagents (Nolan/Scott Biological Laboratories, Inc.) and an "exoantigen identification system" (a kit supplied by Immuno-Mycologics, Inc.) were evaluated at two laboratories for their possible application in the microimmunodiffusion test to identify Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma species. Both laboratories accurately identified all the cultures, and comparison of the results obtained with the commercial products and those established with the conventional methods performed by a third control laboratory demonstrated full agreement. The use of these two commercial products is recommended for the immunologic identification of cultures suspected of being B. dermatitidis, C. immitis, and Histoplasma species. It was observed, however, that the exoantigen reactions for four B. dermatitidis and two C. immitis isolates were much sharper and more readable with the individual reagents than the reactions observed with the kit.
在两个实验室对市售的单个血清学试剂（诺兰/斯科特生物实验室公司）和一种“外抗原鉴定系统”（免疫真菌学公司提供的试剂盒）进行了评估，以确定它们在微量免疫扩散试验中用于鉴定皮炎芽生菌、粗球孢子菌和组织胞浆菌属的可能性。两个实验室都准确鉴定了所有培养物，将使用商业产品获得的结果与由第三个对照实验室采用传统方法得出的结果进行比较，结果完全一致。推荐使用这两种商业产品对疑似皮炎芽生菌、粗球孢子菌和组织胞浆菌属的培养物进行免疫学鉴定。然而，观察到对于4株皮炎芽生菌和2株粗球孢子菌分离株，使用单个试剂进行外抗原反应比使用试剂盒观察到的反应更清晰、更易于判读。
