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基于农艺和叶片表型性状的艾蒿种质资源多样性

[Diversity of Artemisia argyi germplasm resources based on agronomic and leaf phenotypic traits].

作者信息

Chen Chang-Jie, Luo Dan-Dan, Miao Yu-Huan, Guo Lan-Ping, Liu Da-Hui

机构信息

Pharmacy Faculty, Hubei University of Chinese Medicine Wuhan 430065, China.

Pharmacy Faculty, Hubei University of Chinese Medicine Wuhan 430065, China State Key Laboratory Breeding Base of Dao-di Herbs, National Rresource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2021 Jun;46(11):2773-2782. doi: 10.19540/j.cnki.cjcmm.20210125.101.

Abstract

In this study, in order to evaluate the phenotypic diversity of Artemisia argyi germplasm resources and improve its efficiency of cultivation and breeding, 100 accessions of A. argyi germplasm resources from 58 regions in China were collected, 20 agronomic traits and leaf phenotypic traits were observed and described. The data were used for phenotypic diversity analysis, correlation analysis, principal component analysis and cluster analysis. The result showed that the genetic diversity index of 20 traits ranged from 0.82 to 4.37, among which the largest was the base depth and the smallest was the leaf width; the coefficient of variation of the 12 quantitative traits ranged from 10.55% to 41.47%. the highest coefficient of variation was the height of dead leaves, and the smallest was the content of chlorophyll, except for the angle of branches, all the quantitative characters tended to be normal distribution. The correlation analysis showed that 28 pairs of traits had significant correlation(P<0.01), and 13 pairs had significant correlation(P<0.05). According to principal component analysis, 20 traits were simplified into 9 principal components, and the cumulative contribution rate was 73.414%, nine traits including plant height, dead leaves heigh, stem diameter, symmetry of leave base, stipule, leaf tip shape, depth of the first pair of lobes, depth of the second pair of lobes and leaf yield were selected as key indexes for evaluating agronomic traits and leaf phenotypic traits of A.argyi germplasm resources. With cluster analysis, 100 accessions of A.argyi were classified into 3 groups, the groupⅠincluded the dwarf plants with thick stem and large leaf, the groupⅡincluded high plants with wide leaf and high yield, the group Ⅲ included dwarf plants with thin stem and flat bottom shape of leaf, which could provide the basis for cultivation identification and variety breeding of A.argyi germplasm resources.

摘要

本研究为评价艾蒿种质资源的表型多样性,提高其栽培与育种效率,收集了来自中国58个地区的100份艾蒿种质资源,观察并描述了20个农艺性状和叶片表型性状。将这些数据用于表型多样性分析、相关性分析、主成分分析和聚类分析。结果表明,20个性状的遗传多样性指数在0.82至4.37之间,其中最大的是基部深度,最小的是叶宽;12个数量性状的变异系数在10.55%至41.47%之间,变异系数最高的是枯叶高度,最小的是叶绿素含量,除分枝角度外,所有数量性状均呈正态分布。相关性分析表明,28对性状具有极显著相关性(P<0.01),13对性状具有显著相关性(P<0.05)。通过主成分分析,将20个性状简化为9个主成分,累积贡献率为73.414%,选取株高、枯叶高度、茎粗、叶基部对称性、托叶、叶尖形状、第一对裂片深度、第二对裂片深度和叶产量9个性状作为评价艾蒿种质资源农艺性状和叶片表型性状的关键指标。通过聚类分析,将100份艾蒿种质分为3组,第Ⅰ组包括茎粗叶大的矮化植株,第Ⅱ组包括叶宽高产的高植株,第Ⅲ组包括茎细叶基部扁平的矮化植株,可为艾蒿种质资源的栽培鉴定和品种选育提供依据。

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