Luo Dandan, Zeng Zeyi, Wu Zongqi, Chen Changjie, Zhao Tingting, Du Hongzhi, Miao Yuhuan, Liu Dahui
College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065 China.
3 Biotech. 2023 Feb;13(2):57. doi: 10.1007/s13205-022-03412-y. Epub 2023 Jan 22.
Different collections and accessions of (Chinese mugwort) harbour considerable diversity in morphology and bioactive compounds, but no mechanisms have been reported that explain these variations. We studied genome size in accessions from different regions of China by flow cytometry. Genome size was significantly distinct among origins of these 42 Chinese mugwort accessions, ranging from 8.428 to 11.717 pg. There were no significant intraspecific differences among the 42 accessions from the five regions of China. The clustering analysis showed that these 42 accessions could be divided into three groups, which had no significant relationship with geographical location. In a genome survey, the total genome size of (A15) was estimated to be 7.852 Gb (or 8.029 pg) by -mer analysis. This indicated that the results from the two independent methods are consistent, and that the genome survey can be used as an adjunct to flow cytometry to compensate for its deficiencies. In addition, genome survey can provide the information about heterozygosity, repeat sequences, GC content and ploidy of genome. The nuclear DNA contents determined here provide a new reference for intraspecific variation in genome size in and may also be a potential resource for the study of genetic diversity and for breeding new cultivar.
不同的艾蒿（中国艾草）收集品和种质在形态和生物活性化合物方面具有相当大的多样性，但尚未有报道解释这些变异的机制。我们通过流式细胞术研究了来自中国不同地区的艾蒿种质的基因组大小。这42份中国艾蒿种质的基因组大小在不同来源之间存在显著差异，范围从8.428皮克到11.717皮克。来自中国五个地区的42份种质之间没有显著的种内差异。聚类分析表明，这42份艾蒿种质可分为三组，与地理位置没有显著关系。在基因组调查中，通过k-mer分析估计艾蒿（A15）的基因组总大小为7.852Gb（或8.029皮克）。这表明两种独立方法的结果是一致的，并且基因组调查可以作为流式细胞术的辅助手段来弥补其不足。此外，基因组调查可以提供关于艾蒿基因组杂合性、重复序列、GC含量和倍性的信息。此处测定的核DNA含量为艾蒿基因组大小的种内变异提供了新的参考，也可能是研究遗传多样性和培育新品种的潜在资源。
