在糖尿病高同型半胱氨酸血症中，Hsa_circRNA_102682 的表达水平降低与肌酸酐呈负相关，与 TGF-β和 CTGF 有关。
Lower expression of Hsa_circRNA_102682 in diabetic hyperhomocysteinemia negatively related to creatinemia is associated with TGF-β and CTGF.
机构信息
Diabetes Research Center, School of Medicine, Ningbo University, Ningbo, China.
Zhejiang Provincial Key Laboratory of Pathophysiology, Department of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, Ningbo, China.
出版信息
J Clin Lab Anal. 2021 Aug;35(8):e23860. doi: 10.1002/jcla.23860. Epub 2021 Jul 23.
BACKGROUND
Diabetic nephropathy is a kidney disease caused by long-term hyperglycemia. Hsa_circRNA_102682 is related to the pathogenesis of preeclampsia. Preeclampsia is related to hypertension and proteinuria, and diabetic nephropathy is mainly manifested by hypertension and proteinuria. The main pathological change in diabetic nephropathy is glomerular fibrosis.
METHODS
This study used serum samples of patients treated at Li Huili Eastern Hospital, Ningbo, China, from July 10, 2018 to February 15, 2019. We included 73 patients with diabetes and divided them into a normal-homocysteine group and a high-homocysteine group. We selected used quantitative reverse transcriptase-polymerase chain reaction to measure Hsa_circRNA_102682 concentration in the serum. Serum transforming growth factor-beta and connective tissue growth factor levels were tested using ELISA. The Pearson correlation test was used to assess the correlations between Hsa_circRNA_102682, transforming growth factor-beta, connective tissue growth factor, homocysteine, and creatinine.
RESULT
Hsa_circRNA_102682 was significantly lower in diabetic patients with high levels of homocysteine than in those with normal levels of homocysteine, whereas transforming growth factor-beta and connective tissue growth factor levels were higher in diabetic patients with hyperhomocysteinemia. Hsa_circRNA_102682 was negatively correlated with the levels of transforming growth factor-beta, connective tissue growth factor, homocysteine, and creatinine. Transforming growth factor-beta and connective tissue growth factor were both positively correlated with homocysteine and creatinine.
CONCLUSION
Low Hsa_circRNA_102682 was associated with high levels of transforming growth factor-beta and connective tissue growth factor as well as homocysteine and creatinine. These results suggest that Hsa_circRNA_102682 might be related to the pathogenesis of hyperhomocysteinemia in diabetic nephropathy.
背景
糖尿病肾病是一种由长期高血糖引起的肾脏疾病。Hsa_circRNA_102682 与子痫前期的发病机制有关。子痫前期与高血压和蛋白尿有关，而糖尿病肾病主要表现为高血压和蛋白尿。糖尿病肾病的主要病理变化是肾小球纤维化。
方法
本研究使用了中国宁波李惠利东部医院 2018 年 7 月 10 日至 2019 年 2 月 15 日期间治疗的患者的血清样本。我们纳入了 73 例糖尿病患者，并将其分为正常同型半胱氨酸组和高同型半胱氨酸组。我们使用定量逆转录-聚合酶链反应测量血清中 Hsa_circRNA_102682 的浓度。使用 ELISA 检测血清转化生长因子-β和结缔组织生长因子水平。使用 Pearson 相关检验评估 Hsa_circRNA_102682、转化生长因子-β、结缔组织生长因子、同型半胱氨酸和肌酐之间的相关性。
结果
高同型半胱氨酸血症的糖尿病患者的 Hsa_circRNA_102682 水平明显低于同型半胱氨酸水平正常的患者，而高同型半胱氨酸血症的糖尿病患者的转化生长因子-β和结缔组织生长因子水平较高。Hsa_circRNA_102682 与转化生长因子-β、结缔组织生长因子、同型半胱氨酸和肌酐的水平呈负相关。转化生长因子-β和结缔组织生长因子均与同型半胱氨酸和肌酐呈正相关。
结论
低 Hsa_circRNA_102682 与转化生长因子-β和结缔组织生长因子水平升高以及同型半胱氨酸和肌酐水平升高有关。这些结果表明，Hsa_circRNA_102682 可能与糖尿病肾病高同型半胱氨酸血症的发病机制有关。
Zotero 插件