Peng Yuxin, Jiang Lingmin, Seo Jiyoon, Li Zhun, Choe Hanna, Jeong Jae Cheol, Kim Suk Weon, Kim Young-Min, Kim Cha Young, Lee Jiyoung
Korean Collection for Type Cultures (KCTC), Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
Department of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004915.
An aerobic, Gram-stain-negative, weak-motile, short-rod-shaped bacterial strain, designated JBR3-12, was isolated from halophyte plants, and its taxonomic position was investigated by using a polyphasic taxonomic approach. The strain produced a pink pigment on tryptic soy agar and grew optimally at 25 °C, pH 8 and in the presence of 3 % (w/v) NaCl. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain JBR3-12 formed a lineage within the genus and was most closely related to DS-27 (98.0 %) and PB92 (97.6 %). The DNA G+C content of the genome was 41.3 mol%; the whole genome length was 5 426 070 bp. The major fatty acids of JBR3-12 were iso-C, summed feature 3 (comprising C 6 and/or C 7) and iso-C 3-OH. The predominant polar lipid was phosphatidylethanolamine. The predominant quinone was menaquinone-7. Based on its phenotypic, phylogenetic and genotypic features, strain JBR3-12 is proposed to represent a novel species of the genus , for which the name is sp. nov. The type strain is JBR3-12 (=KCTC 82363=NBRC 114901).
从盐生植物中分离出一株需氧、革兰氏染色阴性、运动性较弱、短杆状的细菌菌株，命名为JBR3-12，并采用多相分类方法研究其分类地位。该菌株在胰蛋白胨大豆琼脂上产生粉红色色素，在25 °C、pH 8和3 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析结果表明，菌株JBR3-12在该属内形成一个分支，与DS-27（98.0 %）和PB92（97.6 %）关系最为密切。基因组的DNA G+C含量为41.3 mol%；全基因组长度为5 426 070 bp。JBR3-12的主要脂肪酸为异C、总和特征3（包含C 6和/或C 7）和异C 3-OH。主要极性脂为磷脂酰乙醇胺。主要醌为甲基萘醌-7。基于其表型、系统发育和基因型特征，菌株JBR3-12被提议代表该属的一个新物种，命名为sp. nov.。模式菌株为JBR3-12（=KCTC 82363=NBRC 114901）。
